지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 97 - 105 (9page)
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초록· 키워드
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Dehydration Responsive Element Binding (DREB) gene is one of the essential transcription factors plants use for responding to stress conditions including salinity, drought, and cold stress. The purpose of this study was to isolate the full length and characterize the DREB gene from three different genotypes of sugarcane, wild, commercial cultivar, and interspecific hybrid sugarcane. The length of the gene, designated ScDREB was 789 bp, and coding for a putative polypeptide of 262 amino acid residues. Sequences of the gene were submitted to the GenBank database with accession numbers of KX280722.1, KX280721.1, and KX280719.1 for wild sugarcane, commercial cultivar (KPS94-13), and interspecific hybrid (Biotec2), respectively. In silico characterization indicated that the deduced polypeptide contains a putative nuclear localization signal (NLS) sequence, and a conserved AP2/ERF domain of the DREB family, at 82-140 amino residues. Based on multiple sequence alignment, sequences of the gene from the three sugarcane genotypes were classified in the DREB2 group. Gene expression analysis indicated, that ScDREB2 expression pattern in tested sugarcane was up-regulated by salt stress. When the plants were under 100 mM NaCl stress, relative expressions of the gene in leaves was higher than those in roots. In contrast, under 200 mM NaCl stress, relative expressions of the gene in roots was higher than those in leaves. This is the first report on cloning the full length and characterization, of ScDREB2 gene of sugarcane. Results indicate that ScDREB2 is highly responsive to salt stress.
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참고문헌 (43)
참고문헌 신청
Agarwal P / 2007 / Stress-inducible DREB2A transcription factor from Pennisetum glaucum is a phosphoprotein and its phosphorylation negatively regulates its DNA-binding activity / Mol Genet Genomics 277:189~198
Armas C / 2010 / Hydraulic lift and tolerance to salinity of semiarid species: Consequences for species interactions / Oecologia 162:11~21
Arunin S / 1984 / Ecology and Management of Problem Soils in Asia / Food and Fertilizer Technology Center for the Asian and Pacific Region :336~351
Ba ANN / 2009 / Moses AM(2009)NLStradamus : A simple hidden markov model for nuclear localization signal prediction / BMC Bioinform 10(1):202
Bouaziz D / 2012 / Ectopic expression of dehydration responsive element binding proteins (StDREB2) confers higher tolerance to salt stress in potato / Plant Physiol Biochem 60:98~108
Armas C / 2010 / Hydraulic lift and tolerance to salinity of semiarid species: Consequences for species interactions / Oecologia 162:11~21
Arunin S / 1984 / Ecology and Management of Problem Soils in Asia / Food and Fertilizer Technology Center for the Asian and Pacific Region :336~351
Ba ANN / 2009 / Moses AM(2009)NLStradamus : A simple hidden markov model for nuclear localization signal prediction / BMC Bioinform 10(1):202
Bouaziz D / 2012 / Ectopic expression of dehydration responsive element binding proteins (StDREB2) confers higher tolerance to salt stress in potato / Plant Physiol Biochem 60:98~108
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