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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 391 - 401 (11page)
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Purpose This study was designed to identify novel fusion transcripts (FTs) and their functional significance in colorectal cancer (CRC) lines. Materials and Methods We performed paired-end RNA sequencing of 28 CRC cell lines. FT candidates were identified using TopHat-fusion, ChimeraScan, and FusionMap tools and further experimental validation was conducted through reverse transcription-polymerase chain reaction and Sanger sequencing. FT was depleted in human CRC line and the effects on cell proliferation, cell migration, and cell invasion were analyzed. Results One thousand three hundred eighty FT candidates were detected through bioinformatics filtering. We selected six candidate FTs, including four inter-chromosomal and two intrachromosomal FTs and each FT was found in at least one of the 28 cell lines. Moreover, when we tested 19 pairs of CRC tumor and adjacent normal tissue samples, NFATC3– PLA2G15 FT was found in two. Knockdown of NFATC3–PLA2G15 using siRNA reduced mRNA expression of epithelial–mesenchymal transition (EMT) markers such as vimentin, twist, and fibronectin and increased mesenchymal–epithelial transition markers of E-cadherin, claudin-1, and FOXC2 in colo-320 cell line harboring NFATC3–PLA2G15 FT. The NFATC3–PLA2G15 knockdown also inhibited invasion, colony formation capacity, and cell proliferation. Conclusion These results suggest that that NFATC3–PLA2G15 FTs may contribute to tumor progression by enhancing invasion by EMT and proliferation.
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참고문헌 (30)
참고문헌 신청
Siegel R / 2014 / Colorectal cancer statistics, 2014 / CA Cancer J Clin 64:104~117
Allen TM / 2002 / Ligand-targeted therapeutics in anticancer therapy / Nat Rev Cancer 2:750~763
Maher CA / 2009 / Chimeric transcript discovery by pairedend transcriptome sequencing / Proc Natl Acad Sci U S A 106:12353~12358
Maher CA / 2009 / Transcriptome sequencing to detect gene fusions in cancer / Nature 458:97~101
Ozsolak F / 2011 / RNA sequencing : advances, challenges and opportunities / Nat Rev Genet 12:87~98
Allen TM / 2002 / Ligand-targeted therapeutics in anticancer therapy / Nat Rev Cancer 2:750~763
Maher CA / 2009 / Chimeric transcript discovery by pairedend transcriptome sequencing / Proc Natl Acad Sci U S A 106:12353~12358
Maher CA / 2009 / Transcriptome sequencing to detect gene fusions in cancer / Nature 458:97~101
Ozsolak F / 2011 / RNA sequencing : advances, challenges and opportunities / Nat Rev Genet 12:87~98
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