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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제23권 제4호
- 발행연도
- 2019.1
- 수록면
- 237 - 249 (13page)
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초록· 키워드
오류제보하기
Confirming the direct link between neural circuit activity and animal behavior has been a principal aim of neuroscience. The genetically encoded calcium indicator (GECI), which binds to calcium ions and emits fluorescence visualizing intracellular calcium concentration, enables detection of in vivo neuronal firing activity. Various GECIs have been developed and can be chosen for diverse purposes. These GECI-based signals can be acquired by several tools including two-photon microscopy and microendoscopy for precise or wide imaging at cellular to synaptic levels. In addition, the images from GECI signals can be analyzed with open source codes including constrained non-negative matrix factorization for endoscopy data (CNMF_E) and miniscope 1-photon-based calcium imaging signal extraction pipeline (MIN1PIPE), and considering parameters of the imaged brain regions (e.g., diameter or shape of soma or the resolution of recorded images), the real-time activity of each cell can be acquired and linked with animal behaviors. As a result, GECI signal analysis can be a powerful tool for revealing the functions of neuronal circuits related to specific behaviors.
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참고문헌 (125)
참고문헌 신청
Kawashima T / 2014 / A new era for functional labeling of neurons : activity-dependent promoters have come of age / Front Neural Circuits 8:37
Minatohara K / 2016 / Role of immediate-early genes in synaptic plasticity and neuronal ensembles underlying the memory trace / Front Mol Neurosci 8:78
Cho J / 2015 / Multiple repressive mechanisms in the hippocampus during memory formation / Science 350:82~87
Xiu J / 2014 / Visualizing an emotional valence map in the limbic forebrain by TAI-FISH / Nat Neurosci 17:1552~1559
Zhang Q / 2018 / Use of TAI-FISH to visualize neural ensembles activated by multiple stimuli / Nat Protoc 13:118~133
Minatohara K / 2016 / Role of immediate-early genes in synaptic plasticity and neuronal ensembles underlying the memory trace / Front Mol Neurosci 8:78
Cho J / 2015 / Multiple repressive mechanisms in the hippocampus during memory formation / Science 350:82~87
Xiu J / 2014 / Visualizing an emotional valence map in the limbic forebrain by TAI-FISH / Nat Neurosci 17:1552~1559
Zhang Q / 2018 / Use of TAI-FISH to visualize neural ensembles activated by multiple stimuli / Nat Protoc 13:118~133
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