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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 262 - 269 (8page)
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초록· 키워드
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The porcine myeloid antimicrobial peptide (PMAP), one of the cathelicidin family members, contains small cationic peptides with amphipathic properties. We used a putative lysozyme originated from the bacteriophage P22 (P22 lysozyme) as a fusion partner, which was connected to the N-terminus of the PMAP36 peptide, to markedly increase the expression levels of recombinant PMAP36. The PMAP36-P22 lysozyme fusion protein with high solubility was produced in Escherichia coli. The final purified yield was approximately 1.8 mg/L. The purified PMAP36-P22 lysozyme fusion protein exhibited antimicrobial activity against both Gram-negative and Grampositive bacteria (Staphylococcus aureus, Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa, and Bacillus subtilis). Furthermore, we estimated its hemolytic activity against pig erythrocytes as 6% at the high concentration (128μM) of the PMAP36-P22 lysozyme fusion protein. Compared with the PMAP36 peptide (12%), our fusion protein exhibited half of the hemolytic activity. Overall, our recombinant PMAP36-P22 lysozyme fusion protein sustained the antimicrobial activity with the lower hemolytic activity associated with the synthetic PMAP36 peptide. This study suggests that the PMAP36-P22 lysozyme fusion system could be a crucial addition to the plethora of novel antimicrobials.
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참고문헌 (26)
참고문헌 신청
Bahnsen, J. S. / 2015 / Cell-penetrating antimicrobial peptides-prospectives for targeting intracellular infections / Pharm. Res 32:1546~1556
Bulet, P. / 1993 / A novel inducible antibacterial peptide of Drosophila carries an O-glycosylated substitution / J. Biol. Chem 268:14893~14897
Rui Ding / 2017 / Therapeutic Benefits of Mesenchymal Stromal Cells in a Rat Model of Hemoglobin-Induced Hypertensive Intracerebral Hemorrhage / Molecules and Cells 40(2):133~142
Hancock, R. E. / 2006 / Antimicrobial and hostdefense peptides as new anti-infective therapeutic strategies / Nat. Biotechnol 24:1551~1557
Huang, Y. / 2010 / Alpha-helical cationic antimicrobial peptides : relationships of structure and function / Protein Cell 1:143~152
Bulet, P. / 1993 / A novel inducible antibacterial peptide of Drosophila carries an O-glycosylated substitution / J. Biol. Chem 268:14893~14897
Rui Ding / 2017 / Therapeutic Benefits of Mesenchymal Stromal Cells in a Rat Model of Hemoglobin-Induced Hypertensive Intracerebral Hemorrhage / Molecules and Cells 40(2):133~142
Hancock, R. E. / 2006 / Antimicrobial and hostdefense peptides as new anti-infective therapeutic strategies / Nat. Biotechnol 24:1551~1557
Huang, Y. / 2010 / Alpha-helical cationic antimicrobial peptides : relationships of structure and function / Protein Cell 1:143~152
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