지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 463 - 468 (6page)
이용수
초록· 키워드
오류제보하기
Autosomal dominant polycystic kidney disease (ADPKD), one of the most common human monogenic diseases (frequency of 1/1000-1/400), is characterized by numerous fluid-filled renal cysts (RCs). Inactivation of the PKD1 or PKD2 gene by germline and somatic mutations is necessary for cyst formation in ADPKD. To mechanistically understand cyst formation and growth, we isolated RCs from Korean patients with ADPKD and immortalized them with human telomerase reverse transcriptase (hTERT). Three hTERT-immortalized RC cell lines were characterized as proximal epithelial cells with germline and somatic PKD1 mutations. Thus, we first established hTERT-immortalized proximal cyst cells with somatic PKD1 mutations. Through transcriptome sequencing and Gene Ontology (GO) analysis, we found that upregulated genes were related to cell division and that downregulated genes were related to cell differentiation. We wondered whether the upregulated gene for the chemokine CXCL12 is related to the mTOR signaling pathway in cyst growth in ADPKD. CXCL12 mRNA expression and secretion were increased in RC cell lines. We then examined CXCL12 levels in RC fluids from patients with ADPKD and found increased CXCL12 levels. The CXCL12 receptor CXC chemokine receptor 4 (CXCR4) was upregulated, and the mTOR signaling pathway, which is downstream of the CXCL12/CXCR4 axis, was activated in ADPKD kidney tissue. To confirm activation of the mTOR signaling pathway by CXCL12 via CXCR4, we treated the RC cell lines with recombinant CXCL12 and the CXCR4 antagonist AMD3100; CXCL12 induced the mTOR signaling pathway, but the CXCR4 antagonist AMD3100 blocked the mTOR signaling pathway. Taken together, these results suggest that enhanced CXCL12 in RC fluids activates the mTOR signaling pathway via CXCR4 in ADPKD cyst growth.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Peters DJ / 2001 / Autosomal dominant polycystic kidney disease : modification of disease progression / Lancet 358:1439~1444
Harris PC / 2009 / Polycystic kidney disease / Annu Rev Med 60:321~337
Ghata J / 2017 / Polycystic Kidney Disease / Compr Physiol 7:945~975
Sutters M / 2003 / Autosomal dominant polycystic kidney disease : molecular genetics and pathophysiology / J Lab Clin Med 141:91~101
Watnick TJ / 1997 / An unusual pattern of mutation in the duplicated portion of PKD1 is revealed by use of a novel strategy for mutation detection / Hum Mol Genet 6:1473~1481
Harris PC / 2009 / Polycystic kidney disease / Annu Rev Med 60:321~337
Ghata J / 2017 / Polycystic Kidney Disease / Compr Physiol 7:945~975
Sutters M / 2003 / Autosomal dominant polycystic kidney disease : molecular genetics and pathophysiology / J Lab Clin Med 141:91~101
Watnick TJ / 1997 / An unusual pattern of mutation in the duplicated portion of PKD1 is revealed by use of a novel strategy for mutation detection / Hum Mol Genet 6:1473~1481
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
NF-κB signaling regulates cell-autonomous regulation of CXCL10 in breast cancer 4T1 cells
Experimental and Molecular Medicine
2017 .01
-
Overexpression of CXCL2 inhibits cell proliferation and promotes apoptosis in hepatocellular carcinoma
BMB Reports
2018 .01
-
Rapamycin-resistant and torin-sensitive mTOR signaling promotes the survival and proliferation of leukemic cells
BMB Reports
2016 .01
-
Assessment of combinational effect of HCA and other dietary supplements on MDCK cyst for possible ADPKD treatment
한국고분자학회 학술대회 연구논문 초록집
2017 .10
-
Production of CXCR2 in E. coli and Reconstitution of Its Molecular Structure Using a Nanodisc
한국생물공학회 학술대회
2021 .10
-
Pharmacological inhibition of mTOR attenuates replicative cell senescence and improves cellular function via regulating the STAT3-PIM1 axis in human cardiac progenitor cells
Experimental and Molecular Medicine
2020 .01
-
Effects of PEP-1-FK506BP on cyst formation in polycystic kidney disease
BMB Reports
2017 .01
-
Functional Reconstitution of Chemokine Receptors in Nanodisc and Their Characterization
한국생물공학회 학술대회
2022 .09
-
Long and Short-Term Effect of mTOR Regulation on Cerebral Organoid Growth and Differentiations
조직공학과 재생의학
2024 .01
-
The role of amino acid-induced mammalian target of rapamycin complex 1(mTORC1) signaling in insulin resistance
Experimental and Molecular Medicine
2016 .01
-
Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury
Experimental and Molecular Medicine
2022 .11
-
Cell Cycle Arrest Effects of Extract from Artemisia annuain through Akt/mTOR Signaling Pathway in HCT116 Colon Cancer Cells
한국생물공학회 학술대회
2015 .04
-
Investigation of Natural Killer Cell-tumor Interaction in Dynamic Tumor-on-a chip
한국생물공학회 학술대회
2023 .10
-
Extracts fromCinnamomumcamphorainduce G1 Cell Cycle Arrest in HCT116 Colon Cancer Cells via Regulating of Akt/mTOR/p53 signaling Pathway
한국생물공학회 학술대회
2018 .04
-
Tubeimoside-1 suppresses breast cancer metastasis through downregulation of CXCR4 chemokine receptor expression
BMB Reports
2016 .01
-
Comparative global immune-related gene profiling of somatic cells, human pluripotent stem cells and their derivatives: implication for human lymphocyte proliferation
Experimental and Molecular Medicine
2017 .01
-
Introduction of Telomerase Reverse Transcriptase Gene into Epidermal Stem Cells Derived from Human Skins
조직공학과 재생의학
2015 .01
-
RC부재의 R-μ-T의 통계적 특성 개발
한국콘크리트학회 학술대회 논문집
2024 .11
-
RCS 표준화 동향 연구
한국통신학회 학술대회논문집
2024 .11
-
Atorvastatin Pretreatment Ameliorates Mesenchymal Stem Cell Migration through miR-146a/CXCR4 Signaling
조직공학과 재생의학
2021 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0