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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 245 - 261 (17page)
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The SnRK (sucrose non-fermenting-1 related protein kinase) families respond to salt tolerance, nutritional stress, disease stress, and carbon metabolic regulation. In this study, the SnRK2.8 gene was cloned from Arabidopsis thaliana and nominated AtSnRK2.8. Bioinformatics characterization of AtSnRK2.8 showed that it contained an ATP binding site and a serine/ threonine protein kinase binding site at the N-terminal catalytic domain. In addition, the AtSnRK2.8 protein contained special domain I at the C-terminal catalytic domain. Real-time polymerase chain reaction (PCR) results showed that the AtSnRK2.8 gene was expressed in all tested tissues and was highly expressed in seeds. Our analyses proved that the AtSnRK2.8 gene expression was upregulated by salt and drought stresses. The recombinant AtSnRK2.8 protein was expressed using a pET-28a system and purified with HisTrap HP affinity columns. The recombinant AtSnRK2.8 protein has the properties of a protein kinase. The results of overexpressing AtSnRK2.8 in Nanlin895 poplar showed that the transgenic poplar plants had a better ability to resist drought and salt stress compared to the wild type, and adapted well to drought and a high-salt environment.
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참고문헌 (40)
참고문헌 신청
Alderson A / 1991 / Complementation of snf1, a mutation affecting global regulation of carbon metabolism in yeast, by a plant protein kinase cDNA / Proc Natl Acad Sci 88(19):8602~8605
Allen JA / 1994 / Prospects for increasing the salt tolerance of forest trees: a review / Tree Physiol 14(7_9):843
Anderberg RJ / 1992 / Isolation of a wheat cdna clone for an abscisic acid-inducible transcript with homology to protein kinases / Proc Natl Acad Sci USA 89(21):10183~10187
Boudsocq M / 2004 / Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana / J Biol Chem 279(40):41758~41766
Cheeke TE / 2012 / Microbial ecology in sustainable agroecosystems / CRC Press :153~227
Allen JA / 1994 / Prospects for increasing the salt tolerance of forest trees: a review / Tree Physiol 14(7_9):843
Anderberg RJ / 1992 / Isolation of a wheat cdna clone for an abscisic acid-inducible transcript with homology to protein kinases / Proc Natl Acad Sci USA 89(21):10183~10187
Boudsocq M / 2004 / Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana / J Biol Chem 279(40):41758~41766
Cheeke TE / 2012 / Microbial ecology in sustainable agroecosystems / CRC Press :153~227
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