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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 475 - 481 (7page)
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The evolution of genome editing technology based on CRISPR (clustered regularly interspaced short palindromic repeats) system has led to a paradigm shift in biological research. CRISPR/Cas9-guide RNA complexes enable rapid and efficient genome editing in mammalian cells. This system induces double-stranded DNA breaks (DSBs) at target sites and most DNA breakages induce mutations as small insertions or deletions (indels) by non-homologous end joining (NHEJ) repair pathway. However, for more precise correction as knock-in or replacement of DNA base pairs, using the homology-directed repair (HDR) pathway is essential. Until now, many trials have greatly enhanced knock-in or substitution efficiency by increasing HDR efficiency, or newly developed methods such as Base Editors (BEs). However, accuracy remains unsatisfactory. In this review, we summarize studies to overcome the limitations of HDR using the CRISPR system and discuss future direction.
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참고문헌 (70)
참고문헌 신청
Wang H / 2013 / One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering / Cell 153:910~918
Yang H / 2013 / One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering / Cell 154:1370~1379
Barrangou R / 2007 / CRISPR provides acquired resistance against viruses in prokaryotes / Science 315:1709~1712
Horvath P / 2010 / CRISPR/Cas, the immune system of bacteria and archaea / Science 327:167~170
Terns MP / 2011 / CRISPR-based adaptive immune systems / Curr Opin Microbiol 14:321~327
Yang H / 2013 / One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering / Cell 154:1370~1379
Barrangou R / 2007 / CRISPR provides acquired resistance against viruses in prokaryotes / Science 315:1709~1712
Horvath P / 2010 / CRISPR/Cas, the immune system of bacteria and archaea / Science 327:167~170
Terns MP / 2011 / CRISPR-based adaptive immune systems / Curr Opin Microbiol 14:321~327
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Structural and dynamic views of the CRISPR-Cas system at the single-molecule level
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RNA-targeting CRISPR-Cas Effector as a Synthetic Gene Knockdown Tool in Bacterial Cells
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Development of DNA-Free Genome Editing Technology for No Dextran Producing Leuconostoc citreum Using CRISPR-Cas9 System
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