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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 179 - 185 (7page)
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Pluripotent stem cells (PSCs) are a useful source of cells for exploring the role of genes related with early developmental processes and specific diseases due to their ability to differentiate into all somatic cell types. Recently, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) protein 9 system has proven to be a robust tool for targeted genetic modification. Here, we generated miR-451-deficient PSCs using the CRISPR/Cas9 system with PCR-based homologous recombination donor and investigated the impact of its deletion on self-renewal and hematopoietic development. CRISPR/Cas9-mediated miR-451 knockout did not alter the gene expressions of pluripotency, cellular morphology, and cell cycle, but led to impaired erythrocyte development. These findings propose that a combination of PSCs and CRISPR/Cas9 system could be useful to promote biomedical applications of PSCs by elucidating the function and manipulating of specific miRNAs during lineage specification and commitment.
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참고문헌 (36)
참고문헌 신청
Romito A / 2016 / Pluripotent stem cells: current understanding and future directions / Stem Cells Int
Ireland RG / 2015 / Human pluripotent stem cell mechanobiology : manipulating the biophysical microenvironment for regenerative medicine and tissue engineering applications / Stem Cells 33:3187~3196
Bartel DP / 2004 / MicroRNAs : genomics, biogenesis, mechanism, and function / Cell 116:281~297
Lee YJ / 2016 / Dissecting microRNA-mediated regulation of stemness, reprogramming, and pluripotency / Cell Regen(Lond) 5:2
Hong Lian Jin / 2012 / Dynamic Expression of Specific miRNAs during Erythroid Differentiation of Human Embryonic Stem Cells / Molecules and Cells 34(2):177~183
Ireland RG / 2015 / Human pluripotent stem cell mechanobiology : manipulating the biophysical microenvironment for regenerative medicine and tissue engineering applications / Stem Cells 33:3187~3196
Bartel DP / 2004 / MicroRNAs : genomics, biogenesis, mechanism, and function / Cell 116:281~297
Lee YJ / 2016 / Dissecting microRNA-mediated regulation of stemness, reprogramming, and pluripotency / Cell Regen(Lond) 5:2
Hong Lian Jin / 2012 / Dynamic Expression of Specific miRNAs during Erythroid Differentiation of Human Embryonic Stem Cells / Molecules and Cells 34(2):177~183
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