지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
In an effort to develop the tools for monitoring the contamination of
xenoestrogen in the aquatic environment of Korea, reverse transcription-polymerase
chain reaction (RT-PCR) analysis of vitellogenin (VTG) mRNA expression were
optimized in Synechogobius hastus. Based on the partial VTG cDNA sequence VTG
mRNA level in livers from male fishes was analyzed by RT-PCR. As an internal control
beta actin mRNA was amplified. 3 μg of total RNA was reverse transcribed in 20 μl
reaction using murine leukemia virus [MuLV] reverse transcriptase. Subsequent PCR
using the 1 μg of cDNA resulted in linear increase in PCR product of VTG in female
liver cDNA from 10 to 30 cycles of amplification. On the contrary, in male, PCR product
first detected at 28 cycles of amplification and linearly increased during 38 cycles of
amplification, suggesting that male S. hastus expresses minute amount of VTG mRNA
which is 2-18 equivalent of female. In conclusion, the optimized protocol of VTG mRNA
expression in the liver of male S. hastus will be promising the environmental monitoring
the xenoestrogen contamination in the western coast and estuaries in Korea.
목차
등록된 정보가 없습니다.
참고문헌 (28)
참고문헌 신청
A Iwata / 1993 / Fishes of Japan withPictorial Keys to the Species : 1079 ~ 1082
Amer MA / 2001 / Involvement of sex steroid hormones in the early stagesof spermatogenesis in Japanese huchen 65 : 1057 ~ 1066
Bowman CJ / 2000 / Estrogen-induced vitellogenin mRNAand protein in sheepshead minnow 120 : 300 ~ 313
Corsi I / 2003 / Cytochrome P450acetylcholinesterase andgonadal histology for evaluating contaminant exposurelevels in fishes from a highly eutrophic brackish ecosystem 46 :
Falandysz J,6 L Strandberg, / 2001 / Chlorinated cyclodiene pesticide residues in blue mussel, crab, and fish in the Gulf of Gdansk, Baltic Sea. 35 : 4163 ~ 4169
Amer MA / 2001 / Involvement of sex steroid hormones in the early stagesof spermatogenesis in Japanese huchen 65 : 1057 ~ 1066
Bowman CJ / 2000 / Estrogen-induced vitellogenin mRNAand protein in sheepshead minnow 120 : 300 ~ 313
Corsi I / 2003 / Cytochrome P450acetylcholinesterase andgonadal histology for evaluating contaminant exposurelevels in fishes from a highly eutrophic brackish ecosystem 46 :
Falandysz J,6 L Strandberg, / 2001 / Chlorinated cyclodiene pesticide residues in blue mussel, crab, and fish in the Gulf of Gdansk, Baltic Sea. 35 : 4163 ~ 4169
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