지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
- To investigate the antifungal activity related protein in pesticidal bacteria,a bacterial strain LTD was isolated from soil collected at Gimje in Jeonbuk province,Korea, and identified as Bacillus subtilis LTD based on a API50 CHB kit and 16S rDNAsequencing. It has an antifungal activity against 9 plant pathogenic fungi in a paperdisc assay. The antifungal activity-deficient mutant, B. subtilis mLTD was induced at a5 kGy dose of 60Co gamma radiation. Using the two -dimensional electrophoresis andthe matrix assisted laser desorption ionization time -of-flight mass spectrometry, thecomparison analysis of proteins between the wild and mutant were performed. A majorintracellular serine proteinase IspA (MW: 32.5 kDa), a NAD (P) H dehydrogenase (MW:20.0 kDa), and a stage II sporulation protein AA, SpoIIAA (MW: 14.3 kDa) were detectedonly in the B. subtilis LTD. These results suggested that the functions of these proteinsfound only in the B. subtilis LTD could be closely related to the antifungal activityagainst plant pathogenic fungi.
목차
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참고문헌 (27)
참고문헌 신청
/ 1991 / Investigation of optimalcondition for foam separation of iturin an antifungalpeptide produced by Bacillus subtilis : 118 ~ 121
Baker KF / 1987 / Evolving concepts of biological control ofplant pathogens : 67 ~ 85
Baum JA / 1995 / Regulation of insecticidalcrystal protein production in Bacillus thuringiensis 18 : 1 ~ 12
Chen FC / 2003 / The IspAprotease’s involvement in the regulation of the sporulationprocess of Bacillus thuringiensis is revealed byproteomic analysis 312 : 708 ~ 715
Cho SJ / 2003 / Detection and characterization of the Gloeosporiumgloeosporioides growth inhibitory compound iturin Afrom Bacillus subtilis strain KS03 : 47 ~ 51
Baker KF / 1987 / Evolving concepts of biological control ofplant pathogens : 67 ~ 85
Baum JA / 1995 / Regulation of insecticidalcrystal protein production in Bacillus thuringiensis 18 : 1 ~ 12
Chen FC / 2003 / The IspAprotease’s involvement in the regulation of the sporulationprocess of Bacillus thuringiensis is revealed byproteomic analysis 312 : 708 ~ 715
Cho SJ / 2003 / Detection and characterization of the Gloeosporiumgloeosporioides growth inhibitory compound iturin Afrom Bacillus subtilis strain KS03 : 47 ~ 51
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