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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제6호
- 발행연도
- 2018.1
- 수록면
- 1,030 - 1,036 (7page)
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Bacillus strains produce various types of antibiotics, and random mutagenesis has traditionally been used to overproduce these natural metabolites. However, this method leads to the accumulation of unwanted mutations in the genome. Here, we rationally designed a single nucleotide substitution in the degU gene to generate a B. subtilis strain displaying increased plipastatin production in a foreign DNA-free manner. The mutant strain (BS1028u) showed improved antifungal activity against Pythium ultimum. Notably, pps operon deletion in BS1028u resulted in complete loss of antifungal activity, suggesting that the antifungal activity strongly depends on the expression of the pps operon. Quantitative real-time PCR and lacZ assays showed that the point mutation resulted in 2-fold increased pps operon expression, which caused the increase in antifungal activity. Likewise, commercial Bacillus strains can be improved to display higher antifungal activity by rationally designed simple modifications of their genome, rendering them more efficient biocontrol agents.
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참고문헌 (24)
참고문헌 신청
Aftab MN / 2012 / Systematic mutagenesis method for enhanced production of bacitracin by Bacillus licheniformis mutant strain UV-MN-HN-6 / Braz. J. Microbiol. 43:78~88
Leonard CA / 2013 / Random mutagenesis of the Aspergillus oryzae genome results in fungal antibacterial activity / Int. J. Microbiol. 2013:901697
Medema MH / 2011 / The future of industrial antibiotic production: from random mutagenesis to synthetic biology / Bioeng. Bugs 2:230~233
Tsuge K / 1999 / The genes degQ, pps, and lpa-8 (sfp) are responsible for conversion of Bacillus subtilis 168 to plipastatin production / Antimicrob. Agents Chemother. 43:2183~2192
Inaoka T / 2009 / ScoC regulates bacilysin production at the transcription level in Bacillus subtilis / J. Bacteriol. 191:7367~7371
Leonard CA / 2013 / Random mutagenesis of the Aspergillus oryzae genome results in fungal antibacterial activity / Int. J. Microbiol. 2013:901697
Medema MH / 2011 / The future of industrial antibiotic production: from random mutagenesis to synthetic biology / Bioeng. Bugs 2:230~233
Tsuge K / 1999 / The genes degQ, pps, and lpa-8 (sfp) are responsible for conversion of Bacillus subtilis 168 to plipastatin production / Antimicrob. Agents Chemother. 43:2183~2192
Inaoka T / 2009 / ScoC regulates bacilysin production at the transcription level in Bacillus subtilis / J. Bacteriol. 191:7367~7371
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