지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 177 - 184 (8page)
이용수
초록· 키워드
오류제보하기
Acid-labile subunit(ALS) is a 85-kDa glycosylated plasma protein which forms a 150-kDa ternary complex with 7.5-kDa insulin-like growth factor(IGF) and 40~45-kDa IGF-binding protein-3. In a previous study, the present authors prepared a porcine ALS(pALS) expression construct by inserting a pALS coding sequence into a plasmid vector following synthesis of the sequence by reverse transcription-polymerase chain reaction(RT-PCR). The expression construct, however, was subsequently found to have a mis-sense mutation at two bases of the pALS coding sequence which is presumed to have occurred through a PCR error. In the present study, the correct coding sequence was synthesized by the site-directed mutagenesis and inserted into the pET-28a(+) plasmid expression vector containing the His-tag sequence flanking the last codon of the insert DNA. After induction of the expression construct in E. coli BL21(DE3) cells, the resulting presumptive recombinant peptide was purified by the Ni-affinity chromatography. Upon SDS- PAGE, the affinity-purified peptide was resolved as a single band at a 66-kDa position which is consistent with the expected molecular mass of the presumptive recombinant pALS. Collectively, results indicate that a recombinant pALS peptide was successfully expressed and purified in the present study.
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Baxter, R. C / 1989 / Binding proteins for the insulin-like growth factors: structure, regulation and function / Prog. Growth Factor Res 1 : 49 ~ 68
Baxter, R. C. / 1989 / High molecular weight insulin-like growth factor binding protein complex / J. Biol. Chem 264 : 11843 ~ 11848
Baxter, R. C. / 1990 / Circulating levels and molecular distribution of the acid-labile(α) subunit of the high molecular weight insulin-like growth factor-binding protein co
Baxter, R. C. / 1994 / Purification and characterization of the acid-labile subunit of rat serum insulin-like growth factor binding protein complex / Endocrinology 134 : 848
Baxter, R. C. / 2002 / Molecular distribution of IGF binding protein-5 in human serum / J. Clin. Endocrinol. Metab 87 : 271 ~ 276
Baxter, R. C. / 1989 / High molecular weight insulin-like growth factor binding protein complex / J. Biol. Chem 264 : 11843 ~ 11848
Baxter, R. C. / 1990 / Circulating levels and molecular distribution of the acid-labile(α) subunit of the high molecular weight insulin-like growth factor-binding protein co
Baxter, R. C. / 1994 / Purification and characterization of the acid-labile subunit of rat serum insulin-like growth factor binding protein complex / Endocrinology 134 : 848
Baxter, R. C. / 2002 / Molecular distribution of IGF binding protein-5 in human serum / J. Clin. Endocrinol. Metab 87 : 271 ~ 276
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
돼지 150-kDa insulin-like growth factor complex의 acid-labile subunit(ALS) 유전자의 intron 및 ALS complementary DNA의 3' 비해독 부위 cloning과 생체조직에서의 ALS 유전자 발현 확인
한국축산학회지
2004 .01
-
대장균과 식물시스템에서 재조합 인간 prominiinsulin 생합성 분석
Journal of Plant Biotechnology
2013 .01
-
Influence of Controlled- and Uncontrolled-pH Operations on Recombinant Phenylalanine Ammonia Lyase Production in Escherichia coli
Food Science and Biotechnology
2009 .08
-
키토산액 침지 처리에 의한 돈육의 저장성 향상 효과
한국식품영양과학회지
2003 .06
-
들깨박단백질로부터 가수분해물과 한외여과 펩타이드 생산을 위한 효소가수분해 조건 및 항산화 활성
Food Science and Preservation
2018 .08
-
반복배열된 토마토 phenylalanine ammonia-Iyase(p AL X1, PAL X2) 유전자의 구조해석
Applied Biological Chemistry
1999 .01
-
Recombinant expression of a novel antimicrobial peptide consisting of human α-defensin 5 and Mytilus coruscus mytilin-1 in Escherichia coli
Applied Biological Chemistry
2015 .01
-
미강 단백질 가수분해물로부터 Ca, Fe 결합된 peptide 제조
Journal of applied biological chemistry
2010 .01
-
Expression of Cyclodextrin Glucanotransferase from Bacillus macerans in Recombinant Escherichia coli
Food Science and Biotechnology
1995 .12
-
Expression and purification of a recombinant antimicrobial peptide from starfish, Patiria pectinifera
한국수산과학회 양식분과 학술대회
2016 .10
-
Immune-enhancing effects of polysaccharides with different molecular weights obtained from Gloiopeltis furcata
Food Science and Preservation
2018 .02
-
Stability of Separated ACE Inhibitory Peptides under Condition of Various pH, Temperature, Gastric Digestion (In Vitro)
한국축산식품학회 학술발표초록집
2005 .01
-
키토산 첨가에 의한 식빵의 저장성 증진효과
한국식품영양과학회지
2002 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0