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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제21권 제2호
- 발행연도
- 2010.1
- 수록면
- 157 - 162 (6page)
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Open reading frame 35 (bm35) of the Bombyx mori nucleopolyhedrovirus (BmNPV) is a special gene whose homologues are only found in some group-I nucleopolyhedroviruses, suggesting that bm35 plays a specific role in the viral life cycle. This paper described the characterization of BmNPV bm35. Computerassisted sequence analysis shows that a putative RING finger motif is observed in the protein, Bm35 encoded by bm35. The coding sequence of bm35 was amplified and subcloned into the vector pET30a(+) and the (His)6-tagged fusion protein His-Bm35 was expressed in the Escherichia coli BL21 (DE3) LysS cells. The bm35 transcript and Bm35 protein were detected in BmNPV-infected BmN cells at 12~48 h post infection (p.i.) by RT-PCR and Western blot analysis using the polyclonal antibody generated by immunizing a rabbit with purified (His)6-tagged Bm35, suggesting that bm35 is synthesized in the late stage of BmNPV infection cycle. Bm35 was not a structural component associated with budded virus (BV) and occlusion derived virus (ODV). These data indicated that bm35 is a functional gene in the BmNPV life cycle.
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참고문헌 (26)
참고문헌 신청
Acharya A / 2002 / Transcriptional analysis and preliminary characterization of ORF Bm42 from Bombyx mori nucleopolyhedrovirus / Virology 299 : 213 ~ 224
Ahrens CH / 1997 / The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome / Virology 229 : 381 ~ 399
Ayres MD / 1994 / The complete DNA sequence of Autographa californica nuclear polyhedrosis virus / Virology 202 : 586 ~ 605
Braunagel SC / 1994 / Autographa californica nuclear polyhedrosis virus, PDV, and ECV viral envelopes and nucleocapsids: structural proteins, antigens, lipid and fatty acid p
Bulach DM / 1999 / Group II nucleopolyhedrovirus subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene sequences / J Invertebr Pathol 73 : 59 ~ 73
Ahrens CH / 1997 / The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome / Virology 229 : 381 ~ 399
Ayres MD / 1994 / The complete DNA sequence of Autographa californica nuclear polyhedrosis virus / Virology 202 : 586 ~ 605
Braunagel SC / 1994 / Autographa californica nuclear polyhedrosis virus, PDV, and ECV viral envelopes and nucleocapsids: structural proteins, antigens, lipid and fatty acid p
Bulach DM / 1999 / Group II nucleopolyhedrovirus subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene sequences / J Invertebr Pathol 73 : 59 ~ 73
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