지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제23권 제1호
- 발행연도
- 2011.1
- 수록면
- 123 - 128 (6page)
이용수
초록· 키워드
오류제보하기
1-Deoxynojirimycin (DNJ) is an alkaloid that is found at relatively high concentrations in mulberry leaf and tissues of the silkworm, Bombyx mori. DNJ is a well known inhibitor of α-glucosidase, an enzyme that is involved in the early stages of the N-linked glycoprotein synthesis pathway. α-Glucosidase activity in the cell extract from B. mori-derived Bm5 cells showed approximately 40-fold less sensitivity to DNJ than α-glucosidase activity in the cell extract from Spodoptera frugiperda-derived Sf9 cells. The replication of B. mori nucleopolyhedrovirus (BmNPV) was not inhibited when it was propagated in BmN cells that were grown in medium containing up to 10 mM DNJ. In contrast,the replication of Autographa californica multiple NPV (AcMNPV) was reduced by 67% when it was propagated in Sf9 cells that were grown in medium containing 10 mM DNJ. The viability of Bm5 and Sf9 cells that were grown in medium containing up to 10 mM DNJ was not affected. Our results suggested that the reduced replication of AcMNPV was the result of the higher sensitivity of α-glucosidase activity in Sf9 cells to DNJ.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Asano N / 2001 / Polyhydroxylated alkaloids isolated from mulberry trees (Morus alba L.) and silkworms (Bombyx mori L.) / J Agric Food Chem 49 : 4208 ~ 4213
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72 : 2
Durantel D / 2001 / Study of the mechanism of antiviral action of iminosugar derivatives against bovine viral diarrhea virus / J Virol 75 : 8987 ~ 8998
Dwek RA / 2002 / Targeting glycosylation as a therapeutic approach / Nat Rev Drug Discov 1 : 65 ~ 75
Francis BR / 2002 / Biosynthesis and processing of Spodoptera frugiperda alpha-mannosidase III / Glycobiology 12 : 369 ~ 377
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72 : 2
Durantel D / 2001 / Study of the mechanism of antiviral action of iminosugar derivatives against bovine viral diarrhea virus / J Virol 75 : 8987 ~ 8998
Dwek RA / 2002 / Targeting glycosylation as a therapeutic approach / Nat Rev Drug Discov 1 : 65 ~ 75
Francis BR / 2002 / Biosynthesis and processing of Spodoptera frugiperda alpha-mannosidase III / Glycobiology 12 : 369 ~ 377
이 논문의 저자 정보
최근 본 자료
댓글(0)
0