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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제17권 제1호
- 발행연도
- 2008.1
- 수록면
- 137 - 141 (5page)
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초록· 키워드
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We previously isolated and cloned a cDNA of abaecin from the Bombus ignitus. In an effort to produce a large amount of soluble abaecin at low cost, we successfully expressed the peptide in Escherichia coli that are highly sensitive to its mature form. For this, we fused the peptide encoding 39 amino acids of mature B. ignitus abaecin to the thioredoxin gene together with a C-terminal 6xHis tag. An enterokinase cleavage site was introduced between the 6xHis tag and mature abaecin to allow final release of the recombinant peptide. A high yield of 9.6 mg soluble fusion protein from 200 ml of bacterial culture was purified by Ni2+-charged His-Bind resin affinity column, and 1.4 mg of pure active recombinant abaecin was readily obtained by enterokinase cleavage, followed by affinity chromatograph. The molecular mass of recombinant abaecin peptide was determined by Tricin-SDS-PAGE analysis. The recombinant abaecin exhibited antibacterial activity against Gram-negative bacteria.
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참고문헌 (12)
참고문헌 신청
Casteels, P. / 1990 / Isolation and characterization of abaecin, a major antibacter al response peptide in the honeybee (Apis mellifera) / Eur. J. Biochem 187 : 381 ~ 386
Choi, Y. S. / 2008 / Cloning and expression profiling of four antibacterial peptide genes form the bumblebee Bombus ignitus / Comp. Biochem. Physiol. B 150 : 141 ~ 146
Hwang, S. W. / 2001 / A simple method for the purification of an antimicrobial peptide in recombinant Escherichia coli / Mol. Biotechnol 18 : 193 ~ 198
Lehrer, R. I. / 1991 / Ultrasensitive assays for endogenous antimicrobial polypeptides / J. Immunol. Methods 137 : 167 ~ 173
Li, Y. / 2006 / Cloning, expression, isotope labeling, and purification of human antimicrobial peptide LL-37 in Escherichia coli for NMR studies / Protein Expr. Purif 47 : 49
Choi, Y. S. / 2008 / Cloning and expression profiling of four antibacterial peptide genes form the bumblebee Bombus ignitus / Comp. Biochem. Physiol. B 150 : 141 ~ 146
Hwang, S. W. / 2001 / A simple method for the purification of an antimicrobial peptide in recombinant Escherichia coli / Mol. Biotechnol 18 : 193 ~ 198
Lehrer, R. I. / 1991 / Ultrasensitive assays for endogenous antimicrobial polypeptides / J. Immunol. Methods 137 : 167 ~ 173
Li, Y. / 2006 / Cloning, expression, isotope labeling, and purification of human antimicrobial peptide LL-37 in Escherichia coli for NMR studies / Protein Expr. Purif 47 : 49
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