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The areas of soybean (Glycine max (L.) Merrill) cultivation in Gangwon-do have increased due to the growing demand for well-being foods. The soybean barcode system is a useful tool for cultivar identification and diversity analysis, which could be used in the seed production system for soybean cultivars. We genotyped cultivars using 202 insertion and deletion (InDel) markers specific to dense variation blocks (dVBs), and examined their ability to identify cultivars and analyze diversity by comparison to the database in the soybean barcode system. The genetic homology of “Cheonga,” “Gichan,” “Daewang,” “Haesal,” and “Gangil” to the 147 accessions was lower than 81.2%, demonstrating that these barcodes have potentiality in cultivar identification. Diversity analysis of one hundred and fifty-three soybean cultivars revealed four subgroups and one admixture (major allele frequency <0.6). Among the accessions, “Heugcheong,” “Hoban,” and “Cheonga” were included in subgroup 1 and “Gichan,” “Daewang,” “Haesal,” and “Gangil” in the admixture. The genetic regions of subgroups 3 and 4 in the admixture were reshuffled for early maturity and environmental tolerance, respectively, suggesting that soybean accessions with new dVB types should be developed to improve the value of soybean products to the end user. These results indicated that the two-dimensional barcodes of soybean cultivars enable not only genetic identification, but also management of genetic resources through diversity analysis.
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참고문헌 (36)
참고문헌 신청
Agarwal M / 2008 / Advances in molecular marker techniques and their applications in plant sciences / Plant Cell Rep 27:617~631
최재근 / 2011 / 장류용 고품질, 대립 콩 신품종 ‘대왕’ / 한국육종학회지 43(3):177~179
Deschamps S / 2010 / Utilization of nextgeneration sequencing platforms in plant genomics and genetic variant discovery / Mol Breed 25:553~570
Ganal MW / 2009 / SNP identification in crop plants / Curr Opin Plant Biol 12:211~217
Evanno G / 2005 / Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study / Mol Ecol 14:2611~2620
최재근 / 2011 / 장류용 고품질, 대립 콩 신품종 ‘대왕’ / 한국육종학회지 43(3):177~179
Deschamps S / 2010 / Utilization of nextgeneration sequencing platforms in plant genomics and genetic variant discovery / Mol Breed 25:553~570
Ganal MW / 2009 / SNP identification in crop plants / Curr Opin Plant Biol 12:211~217
Evanno G / 2005 / Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study / Mol Ecol 14:2611~2620
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