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Fifty-two Korean japonica rice cultivars were analyzed for leaf blast resistance and genotyped with 4 STS and 26 SSR markers flanking the specific chromosome sites linked with blast resistance genes. In our analysis of resistance genes in 52 japonica cultivars using STS markers tightly linked to Pib, Pita, Pi5(t) and Pi9(t), the blast nursery reaction of the cultivars possessing the each four major genes were not identical to that of the differential lines. Eight of the 26 SSR markers were associated with resistant phenotypes against the isolates of blast nursery as well as the specific Korean blast isolates, 90-008 (KI-1113), 03-177 (KJ-105). These markers were linked to Pit, Pish, Pib, Pi5(t), Piz, Pia, Pik, Pi18, Pita and Pi25(t) resistance gene loci. Three of the eight SSR markers, MRG5836, RM224 and RM7102 only showed significantly associated with the phenotypes of blast nursery test for two consecutive years. These three SSR markers also could distinguish between resistant and susceptible japonica cultivars. These results demonstrate the usefulness of marker-assisted selection and genotypic monitoring for blast resistance of rice in blast breeding programs.
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참고문헌 (24)
참고문헌 신청
Causse MA / 1994 / Saturated molecular map of the rice genome based on an interspecific backcross population / Genetics 138 : 1251 ~ 1274
Chen DH / 1999 / Molecular mapping of the rice blast resistance gene, Pi44(t), in a line derived from a durably resistant rice cultivar / Theor Appl Genet 98 : 1046 ~ 1053
Chen X / 1997 / Development of a microsatellite framework map providing genome-wide coverge in rice (Oryza sativa L.) / Theor Appl Genet 95 : 553 ~ 567
Choi JE / 1989 / Resistance of varieties to rice blast in Korea, I. Japonica type of rice varieties / Agri. Sci. Chungnam Natl. Univ 16 : 1 ~ 18
Conaway-Bormans CA / 2003 / Molecular markers linked to the blast resistance gene Pi-z in rice for use in marker-assisted selection / Theor Appl Genet 107 : 1014 ~ 1020
Chen DH / 1999 / Molecular mapping of the rice blast resistance gene, Pi44(t), in a line derived from a durably resistant rice cultivar / Theor Appl Genet 98 : 1046 ~ 1053
Chen X / 1997 / Development of a microsatellite framework map providing genome-wide coverge in rice (Oryza sativa L.) / Theor Appl Genet 95 : 553 ~ 567
Choi JE / 1989 / Resistance of varieties to rice blast in Korea, I. Japonica type of rice varieties / Agri. Sci. Chungnam Natl. Univ 16 : 1 ~ 18
Conaway-Bormans CA / 2003 / Molecular markers linked to the blast resistance gene Pi-z in rice for use in marker-assisted selection / Theor Appl Genet 107 : 1014 ~ 1020
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