지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Many important traits have been tagged allowing plant breeders to apply marker assisted selection (MAS) in rice. PCR itself is simple to set up, and requires little hands-on time. However, a crucial limiting step of MAS programs is the reliable and efficient extraction of DNA which can be performed on thousands of individuals. In this study, We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract DNA from leaf tissues for suitable MAS in rice. We followed the standard 2% CTAB extraction method in all the procedure. In addition we used the 1.2 ml 8-strip tube instead of 1.5 ml E-tubes to fit the 8-multichannel pipette and employ the 96 well plate to use the swing bucket centrifuge. Our modified CTAB DNA extraction method offers several advantages with respect to traditional and simple methods. 1) adult leaf samples collected in paddy field are applicable. 2) 96 leaf samples can be homogenized only one-time by using tungsten carbonate bead and 96well block. 3) semiautomatic loading method using 8-multichannel pipette from DNA extraction to electrophoresis of PCR products. 4) our system can extract about 400 leaf samples per day by only one technicion. Therefore, this method could be useful for marker assisted breeding in rice.
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참고문헌 (13)
참고문헌 신청
Bhattacharjee R / 2003 / An improved semiautomated rapid method of extracting genomic DNA for molecular marker analysis in Cocoa, Theobroma cacao L / Plant Molecular Biology
Chen DH / 1999 / A rapid DNA minipreparation method suitable for AFLP and other PCR applications / Plant Molecular Biology Reporter 17 : 53 ~ 57
Collard BCY / 2007 / Evaluation of quick and dirtyDNA extraction methods for marker -assisted selection in rice (Oryza sativa L.) / Plant Breeding 126 : 47 ~ 50
Guidet F / 1994 / A powerful new technique to quickly prepare hundreds of plant-extracts for PCR and RAPD analyses / Nucleic Acids Res 22 : 1772 ~ 1773
Hayano-Saito Y / 2003 / Chromosomal location of rice panicle blast resistance gene, Pb1, by the analysis using with recombinant inbred lines / The Phytopathological Socieaty
Chen DH / 1999 / A rapid DNA minipreparation method suitable for AFLP and other PCR applications / Plant Molecular Biology Reporter 17 : 53 ~ 57
Collard BCY / 2007 / Evaluation of quick and dirtyDNA extraction methods for marker -assisted selection in rice (Oryza sativa L.) / Plant Breeding 126 : 47 ~ 50
Guidet F / 1994 / A powerful new technique to quickly prepare hundreds of plant-extracts for PCR and RAPD analyses / Nucleic Acids Res 22 : 1772 ~ 1773
Hayano-Saito Y / 2003 / Chromosomal location of rice panicle blast resistance gene, Pb1, by the analysis using with recombinant inbred lines / The Phytopathological Socieaty
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