지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Self-incompatibility (SI) prevents self-fertilization by inhibiting the pollen tube growth of self-pollen. Molecular analysis
has revealed that the S locus comprises a number of genes, such as the S-locus glycoprotein (SLG), the S-locus receptor kinase
(SRK), and SP11 (SCR). Although molecular markers related to those genes have been developed, a simple S-haplotype detecting
method has not been reported due to the highly polymorphic and relatively small coding regions. In this study, the sequence
characterized amplified region (SCAR) markers were used to establish an efficient radish genotyping method. We identified the
S-haplotypes of 192 radish accessions using 19 different markers, which proved to be highly reliable. The accessions were assigned
to 17 types of S-haplotypes, including 8 types of SRKs and 9 types of SLGs. Since the developed SCAR markers are based on
their gene sequences, we could easily identify the S-haplotypes by a single specific band, with the highest frequencies detected
for SLG 5, SRK 1, and SLG 1, in order. Among the tested markers, the SLG 1, SRK 1, and SRK 5 markers exhibited high reliability,
compared to phenotypic results. Furthermore, we identified the seven types of unreported SLGs using SLG Class -I and -II specific
markers. Although the developed SCAR markers still need to be improved for the genotyping of all S-haplotypes, these markers
could be helpful for monitoring inbred lines, and for developing the MAS in radish breeding programs.
목차
등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
Ashizawa M / 1979 / Studies on the breeding of Fusarium resistance in radish / Bull. Veg. Ornam. Crops Res. Stn. Japan. 6 : 39 ~ 70
Bateman AJ / 1955 / Self-incompatibility system in angiosperms. (III) / Cruciferae. Heredity 9 : 52 ~ 68
Doyle JJ / 1990 / Isolation of plant DNA from fresh tissue / Focus 12 : 13 ~ 15
Goring DR / 1992 / The S-locus receptor kinase gene in a self-incompatibility Brassica napus line encodes a functional serine/threonine kinase / Plant Cell 4 : 1273 ~ 1281
Jeong-Pal Seo / 2004 / Identification of S-haplotypes by PCR-RFLP and DNA Sequencing of S-Locus Receptor Kinase (SRK) in Raphanus sativus Inbred Lines / Horticulture, Enviro
Bateman AJ / 1955 / Self-incompatibility system in angiosperms. (III) / Cruciferae. Heredity 9 : 52 ~ 68
Doyle JJ / 1990 / Isolation of plant DNA from fresh tissue / Focus 12 : 13 ~ 15
Goring DR / 1992 / The S-locus receptor kinase gene in a self-incompatibility Brassica napus line encodes a functional serine/threonine kinase / Plant Cell 4 : 1273 ~ 1281
Jeong-Pal Seo / 2004 / Identification of S-haplotypes by PCR-RFLP and DNA Sequencing of S-Locus Receptor Kinase (SRK) in Raphanus sativus Inbred Lines / Horticulture, Enviro
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