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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 232 - 237 (6page)
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Purpose: To evaluate the protective effects of Epigallocatechin gallate (EGCG) against UV irradiation in cultured human retinal pigment epithelial (RPE) cells.
Methods: UV irradiation was produced by a UV lamp for 30 seconds with an irradiance of 3.3 mW/cm2. After 5 minutes and 1 hour, we administered different concentrations of EGCG (0, 5, 10, 15, 25, 50, 100 uM). The cell count was determined under a microscope using a counting chamber and the cell activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.
Results: The cell count of cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group, compared with the non-administrated group. The cell activity of the cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group and was increased in a dose-dependent way as determined by the MTT assay.
Conclusions: The administration of EGCG increased the cell count and the cell activity after UV irradiation in cultured human retinal pigment epithelial cells; this suggests that EGCG provided protection against UV damage in cultured human retinal pigmented epithelial cells.
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참고문헌 (26)
참고문헌 신청
/ / Van Kuijk FJ. Effect of ultraviolet light on the eye role of protective glasses. Environ Health Perspet 1991 : 177 ~ 84
/ 2001 / A randomized, placebo-controlled, clinical trial of high-dose supplementation with vitamins C and E, beta carotene, and zinc for age-related macular degeneration an
Beatty S / 2000 / The role of oxidative stress in the pathogenesis of age-related macular degeneration 45 : 115 ~ 134
Buttemeyer R / 2003 / Epigallocatechin gallate can significantly decrease free oxygen radicals in the reperfusion injury in vivo 35 : 3116 ~ 3120
Hanneken A / 2006 / Flavonoids Protect Human Retinal Pigment Epithelial Cells from Oxidative-Stress-Induced Death 47 : 3164 ~ 3177
/ 2001 / A randomized, placebo-controlled, clinical trial of high-dose supplementation with vitamins C and E, beta carotene, and zinc for age-related macular degeneration an
Beatty S / 2000 / The role of oxidative stress in the pathogenesis of age-related macular degeneration 45 : 115 ~ 134
Buttemeyer R / 2003 / Epigallocatechin gallate can significantly decrease free oxygen radicals in the reperfusion injury in vivo 35 : 3116 ~ 3120
Hanneken A / 2006 / Flavonoids Protect Human Retinal Pigment Epithelial Cells from Oxidative-Stress-Induced Death 47 : 3164 ~ 3177
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