지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제38권 제4호
- 발행연도
- 2011.1
- 수록면
- 216 - 221 (6page)
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초록· 키워드
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Objective: To differentiate the human embryonic stem cells (hESCs) into the retinal pigment epithelium (RPE) in the defined culture condition and determine its therapeutic potential for the treatment of retinal degenerative diseases. Methods: The embryoid bodies were formed from hESCs and attached on the matrigel coated culture dishes. The neural structures consisting neural precursors were selected and expanded to form rosette structures. The mechanically isolated neural rosettes were differentiated into pigmented cells in the media comprised of N2 and B27. Expression profiles of markers related to RPE development were analyzed by reverse transcription-polymerase chain reaction and immunostaining. Dissociated putative RPE cells ($10^5$ cells/5 ${\mu}L$) were transplanted into the subretinal space of rat retinal degeneration model induced by intravenous sodium iodate injection. Animals were sacrificed at 1, 2, and 4 weeks after transplantation, and immnohistochemistry study was performed to verify the survival of the transplanted cells. Results: The putative RPE cells derived from hESC showed characteristics of the human RPE cells morphologically and expressed molecular markers and associated with RPE fate. Grafted RPE cells were found to survive in the subretinal space up to 4 weeks after transplantation, and the expression of RPE markers was confirmed with immunohistochemistry. Conclusion: Transplanted RPE cells derived from hESC in the defined culture condition successfully survived and migrated within subretinal space of rat retinal degeneration model. These results support the feasibility of the hESC derived RPE cells for cell-based therapies for retinal degenerative disease.
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참고문헌 (30)
참고문헌 신청
Zarbin MA / 2004 / Current concepts in the pathogenesis of age-related macular degeneration / Arch Ophthalmol 122:598~614
Strauss O / 2005 / The retinal pigment epithelium in visual function / Physiol Rev 85:845~881
Radtke ND / 2008 / Vision improvement in retinal degeneration patients by implantation of retina together with retinal pigment epithelium / Am J Ophthalmol 146:172~182
Tezel TH / 2007 / Adult retinal pigment epithelial transplantation in exudative age-related macular degeneration / Am J Ophthalmol 143:584~595
Stanga PE / 2002 / Retinal pigment epithelium translocation after choroidal neovascular membrane removal in age-related macular degeneration / Ophthalmology 109:1492~1498
Strauss O / 2005 / The retinal pigment epithelium in visual function / Physiol Rev 85:845~881
Radtke ND / 2008 / Vision improvement in retinal degeneration patients by implantation of retina together with retinal pigment epithelium / Am J Ophthalmol 146:172~182
Tezel TH / 2007 / Adult retinal pigment epithelial transplantation in exudative age-related macular degeneration / Am J Ophthalmol 143:584~595
Stanga PE / 2002 / Retinal pigment epithelium translocation after choroidal neovascular membrane removal in age-related macular degeneration / Ophthalmology 109:1492~1498
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