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학제간연구 과학기술학 뇌인지과학 기술정책 기타 복합학

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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
대한구강악안면외과학회 대한구강악안면외과학회지 대한구강악안면외과학회지 제35권 제6호
발행연도
2009.1
수록면
397 - 402 (6page)

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The purpose of this study is to investigate the effects of alendronate and pamidronate on proliferation and the alkaline phosphatase activity of human bone marrow derived mesenchymal stem cells and to relate the results with bisphosphonate related osteonecrosis of the jaw(BRONJ). With the consent of patients with no systemic disease and undergoing iliac bone graft, cancellous bone was collected to obtain human bone marrow derived mesenchymal stem cells through cell culture. 96 well plate were prepared with a concentration of 104cell/ well. Alendronate and pamidronate were added to each well with the concentration of 10-6M, 10-8M and 10-10M, respectively. Then proliferation capacity of each well was evaluated with the cell counting kit. 24 well plates were prepared with a concentration of 105cell/ml/well and with the bone supplement, alendronate and pamidronate were added with the concentration of 10-6M, 10-8M and 10-10M, respectively on each plate. The plates were cultured for either 24 or 72 hours. Then the cells were sonicated to measure the alkaline phosphatase activity and protein assay was done to standardize the data for analysis. As the concentration of alendronate or pamidronate added to the culture increased, the proliferation capacity of the cells decreased. However, no statistical significance was found between the group with 10-10M of bisphophonate and the control group. Pamidronate was not capable of increasing the alkaline phosphatase activity in all trials. However, alkaline phosphatase activity increased with 24 hours of 10-8M of alendronate treatment and with 48 hours of 10-10M of alendronate treatment. Cell toxicity increased as the bisphosphonate concentration increased. This seems to be associated with the long half life of bisphosphonate, resulting in high concentration of bisphosphonate in the jaw and thus displaying delayed healing after surgical procedures. Alendronate has shown to increase the alkaline phophatase activity of human bone marrow derived mesenchymal stem cells. However, this data is insufficient to conclude that alendronate facilitates the differentiation of human bone marrow derived mesenchymal stem cells. Further studies on DNA level and animal studies are required to support these results.
#Bisphosphonate #Cell proliferation #Alkaline phosphatase #Osteonecrosis #Mesenchymal stem cells

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참고문헌 (24)

참고문헌 신청
Cecchini MG / 1987 / Effect of bisphosphonates on proliferation and viability of mouse bone marrowderived macrophages / J Bone Miner Res 2 : 135 ~ 142 google schola Chavassieux PM / 1997 / Histomorphometric assessment of the long-term effects of alendronate on bone quality and remodeling in patients with osteoporosis / J Clin Invest 100 google schola Cremers S / 2002 / A pharmacokinetic and pharmacodynamic model for intravenous bisphosphonate(pamidronate) in osteoporosis / Eur J Clin Pharmacol 57 : 883 ~ 890 google schola Devogelaer JP / 2002 / New uses of bisphosphonates : osteohenesis imperfecta / Curr Opin Pharmacol 2 : 748 ~ 753 google schola Fisher JE / 1999 / Alendronate mechanism of action : geranylgeraniol, an intermediate in the mevalonate pathway, prevents inhibition of osteoclast formation, bone resoprtion google schola

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