지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한핵의학회 Nuclear medicine and molecular imaging : NMMI Nuclear medicine and molecular imaging : NMMI 제46권 제2호
- 발행연도
- 2012.1
- 수록면
- 81 - 88 (8page)
이용수
초록· 키워드
오류제보하기
Purpose : This study was performed to evaluate the effect of $^{111}In$-labeling on the cell growth, cycle and viability of bone marrow mesenchymal stem cells (BMSCs). Methods : Rat BMSCs were labeled with various doses of $^{111}In$ (0.4-11.1 Bq/cell). The growth curve of $^{111}In$-BMSCs was obtained up to 14th day of labeling. The cell cycle was evaluated by 5-bromo-2-deoxyuridine (BrdU) labeling or propidium iodide (PI) staining. Senescent cells were counted under a light microscope after staining with 5-bromo-4-chloro-3-indolyl-D-galactopyranoside. Flow cytometry was performed to measure apoptotic and necrotic fractions after staining with annexin V-FITC and PI. Results : The growth of BMSCs labeled with higher doses of $^{111}In$ (4.4 or 11.1 Bq/cell) was significantly inhibited from the 3rd day of labeling. Flow cytometry revealed less BrdU-positive BMSCs at 11.1 Bq $^{111}In$/cell during all measurement days and G1 arrest at 4.4 and 11.1 Bq $^{111}In$/cell. Significant increases in apoptosis and necrosis were also observed at 4.4 (3.04%/1.35%) and 11.1 Bq $^{111}In$/cell (9.07%/3.18%) on the 14th day (control=1.60%/0.39%). However, no cellular senescence was visualized up to the 14th day. Conclusion : A high dose of $^{111}In$-labeling induced cell cycle arrest and death in BMSCs; therefore, it should be used with a careful dosimetry in case of applying it to humans.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청이 논문의 저자 정보
최근 본 자료
댓글(0)
0