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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제10호
- 발행연도
- 2010.1
- 수록면
- 1,415 - 1,423 (9page)
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초록· 키워드
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An extracellular xylanase was purified to homogeneity by sequential chromatography of Fomitopsis pinicola culture supernatants on a DEAE-Sepharose column, a gel filtration column, and then on a MonoQ column with fast protein liquid chromatography. The relative molecular mass of the F. pinicola xylanase was determined to be 58 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis and by size-exclusion chromatography, indicating that the enzyme is a monomer. The hydrolytic activity of the xylanase had a pH optimum of 4.5 and a temperature optimum of 70oC.
The enzyme showed a t1/2 value of 33 h at 70oC and catalytic efficiency (kcat=77.4 s-1, kcat/Km=22.7 mg/ml/s) for oatspelt xylan. Its internal amino acid sequences showed a significant homology with hydrolases from glycoside hydrolase (GH)family 10, indicating that the F. pinicola xylanase is a member of GH family 10.
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참고문헌 (24)
참고문헌 신청
Baldrian, P / 2008 / Degradation of cellulose by basidiomycetous fungi / FEMS Microbiol. Rev 32 : 501 ~ 521
Baraznenok, V. A / 1999 / Characterization of neutral xylanases from Chaetomium cellulolyticum and their biobleaching effect on eucalyptus pulp / Enzyme Microb. Technol 25 :
Bhat, M. K / 2001 / Enzymology and other characteristics of cellulases and xylanases in Enzymes in Farm Animal Nutrition / CAB International : 11 ~ 57
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Bruins, M. E / 2001 / Thermozymes and their applications: A review of recent literature and patents / Appl. Biochem. Biotechnol 90 : 155 ~ 186
Baraznenok, V. A / 1999 / Characterization of neutral xylanases from Chaetomium cellulolyticum and their biobleaching effect on eucalyptus pulp / Enzyme Microb. Technol 25 :
Bhat, M. K / 2001 / Enzymology and other characteristics of cellulases and xylanases in Enzymes in Farm Animal Nutrition / CAB International : 11 ~ 57
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Bruins, M. E / 2001 / Thermozymes and their applications: A review of recent literature and patents / Appl. Biochem. Biotechnol 90 : 155 ~ 186
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