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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제10호
- 발행연도
- 2010.1
- 수록면
- 1,424 - 1,429 (6page)
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A poly(γ-glutamic acid) (γPGA)-cholesterol conjugate was synthesized and its properties were then evaluated. The conjugate exhibited an amphiphilic nature derived from the hydrophilic γPGA backbone and the hydrophobic cholesterol side chain. The conjugate spontaneously formed nanoparticles, becoming an aqueous solution when at low concentrations, and at high concentrations the result was the formation of a physical gel. By utilizing the selfaggregating properties of the conjugate in water, an artificial chaperone was developed. A complex of protein,with the nanoparticles of the conjugate, was formed and the protein was released upon the dissociation of the nanoparticles through the addition of β-cyclodextrin. For denatured carbonic anhydrase, the activity was recovered in the artificial chaperone of the nanoparticle conjugate.
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참고문헌 (22)
참고문헌 신청
Akiyoshi, K / 1999 / Molecular chaperone-like activity of hydrogel nanoparticles of hydrophobized pullulan: Thermal stabilization with refolding of carbonic anhydrase B / Bio
Astafieva, I / 1993 / Critical micellization phenomena in block polyelectrolyte solutions / Macromolecules 26 : 7339 ~ 7352
Cavalieri, F / 2007 / Chaperone-like activity of nanoparticles of hydrophobized poly(vinyl alcohol) / Soft Mater 3 : 718 ~ 724
Clark, E. D. B / 1998 / Refolding of recombinant proteins / Curr. Opin. Biotechnol 9 : 157 ~ 163
Cleland, J. L / 1992 / Polyethylene glycol enhanced refolding of bovine carbonic anhydrase B / J. Biol. Chem 267 : 13327 ~ 13334
Astafieva, I / 1993 / Critical micellization phenomena in block polyelectrolyte solutions / Macromolecules 26 : 7339 ~ 7352
Cavalieri, F / 2007 / Chaperone-like activity of nanoparticles of hydrophobized poly(vinyl alcohol) / Soft Mater 3 : 718 ~ 724
Clark, E. D. B / 1998 / Refolding of recombinant proteins / Curr. Opin. Biotechnol 9 : 157 ~ 163
Cleland, J. L / 1992 / Polyethylene glycol enhanced refolding of bovine carbonic anhydrase B / J. Biol. Chem 267 : 13327 ~ 13334
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