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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제6호
- 발행연도
- 2016.1
- 수록면
- 1,124 - 1,131 (8page)
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초록· 키워드
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In this study, the global proteome of the IPEC-J2 cell line was evaluated using ultra-high performance liquid chromatography coupled to a quadrupole Q Exactive™ Orbitrap mass spectrometer. Proteins were isolated from highly confluent IPEC-J2 cells in biological replicates and analyzed by label-free mass spectrometry prior to matching against a porcine genomic dataset. The results identified 1,517 proteins, accounting for 7.35% of all genes in the porcine genome. The highly abundant proteins detected, such as actin, annexin A2, and AHNAK nucleoprotein, are involved in structural integrity, signaling mechanisms, and cellular homeostasis. The high abundance of heat shock proteins indicated their significance in cellular defenses, barrier function, and gut homeostasis. Pathway analysis and annotation using the Kyoto Encyclopedia of Genes and Genomes database resulted in a putative protein network map of the regulation of immunological responses and structural integrity in the cell line. The comprehensive proteome analysis of IPEC-J2 cells provides fundamental insights into overall protein expression and pathway dynamics that might be useful in cell adhesion studies and immunological applications.
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등록된 정보가 없습니다.
참고문헌 (48)
참고문헌 신청
Agle KA / 2011 / Chemokine stimulation promotes enterocyte migration through lamininspecific integrins / Am. J. Physiol. Gastrointest. Liver Physiol 301:G968~G980
Bendtsen JD / 2004 / Feature-based prediction of non-classical and leaderless protein secretion / Protein Eng. Des. Sel 17:349~356
Berschneider HM / 1989 / Development of normal cultured small intestinal epithelial cell lines which transport Na and Cl / Gastroenterology 96:A41
Brosnahan AJ / 2012 / Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations / Vet. Microbiol 156:229~237
Chen D / 2014 / Dual role of MAPK pathway in the regulation of intestinal barrier function / Inflamm. Bowel Dis 20:E16
Bendtsen JD / 2004 / Feature-based prediction of non-classical and leaderless protein secretion / Protein Eng. Des. Sel 17:349~356
Berschneider HM / 1989 / Development of normal cultured small intestinal epithelial cell lines which transport Na and Cl / Gastroenterology 96:A41
Brosnahan AJ / 2012 / Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations / Vet. Microbiol 156:229~237
Chen D / 2014 / Dual role of MAPK pathway in the regulation of intestinal barrier function / Inflamm. Bowel Dis 20:E16
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