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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제11호
- 발행연도
- 2019.1
- 수록면
- 1,817 - 1,829 (13page)
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Porcine deltacoronavirus (PDCoV) is an emerging swine enteric coronavirus that causes diarrhea in neonatal piglets. Like other coronaviruses, PDCoV encodes at least three accessory or species-specific proteins; however, the biological roles of these proteins in PDCoV replication remain undetermined. As a first step toward understanding the biology of the PDCoV accessory proteins, we established a stable porcine cell line constitutively expressing the PDCoV NS7 protein in order to investigate the functional characteristics of NS7 for viral replication. Confocal microscopy and subcellular fractionation revealed that the NS7 protein was extensively distributed in the mitochondria. Proteomic analysis was then conducted to assess the expression dynamics of the host proteins in the PDCoV NS7-expressing cells. Highresolution two-dimensional gel electrophoresis initially identified 48 protein spots which were differentially expressed in the presence of NS7. Seven of these spots, including two upregulated and five down-regulated protein spots, showed statistically significant alterations, and were selected for subsequent protein identification. The affected cellular proteins identified in this study were classified into functional groups involved in various cellular processes such as cytoskeleton networks and cell communication, metabolism, and protein biosynthesis. A substantial down-regulation of α-actinin-4 was confirmed in NS7-expressing and PDCoV-infected cells. These proteomic data will provide insights into the understanding of specific cellular responses to the accessory protein during PDCoV infection.
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참고문헌 (72)
참고문헌 신청
Woo PC / 2012 / Discovery of seven novel mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of gammacoronavirus and deltacoronavirus / J. Virol 86:3995~4008
Li G / 2014 / Full-length genome sequence of porcine deltacoronavirus strain USA/IA/2014/8734 / Genome Announc 2:e00278-14
Marthaler D / 2014 / Complete genome sequence of strain SDCV/USA/Illinois121/2014, a porcine deltacoronavirus from the United States / Genome Announc 2:e00218-14
Marthaler D / 2014 / Rapid detection, complete genome sequencing, and phylogenetic analysis of porcine deltacoronavirus / Emerg. Infect. Dis 20:1347~1350
Wang L / 2014 / Detection and genetic characterization of deltacoronavirus in pigs, Ohio, USA, 2014 / Emerg. Infect. Dis 20:1227~1230
Li G / 2014 / Full-length genome sequence of porcine deltacoronavirus strain USA/IA/2014/8734 / Genome Announc 2:e00278-14
Marthaler D / 2014 / Complete genome sequence of strain SDCV/USA/Illinois121/2014, a porcine deltacoronavirus from the United States / Genome Announc 2:e00218-14
Marthaler D / 2014 / Rapid detection, complete genome sequencing, and phylogenetic analysis of porcine deltacoronavirus / Emerg. Infect. Dis 20:1347~1350
Wang L / 2014 / Detection and genetic characterization of deltacoronavirus in pigs, Ohio, USA, 2014 / Emerg. Infect. Dis 20:1227~1230
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