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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제12호
- 발행연도
- 2015.1
- 수록면
- 2,153 - 2,159 (7page)
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초록· 키워드
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Mycoplasma hyopneumoniae is known to cause porcine enzootic pneumonia (EP), an important disease in swine production. The objective of this study was to examine the effects of sonicated protein fractions of M. hyopneumoniae on inflammatory response and gene expression in the murine alveolar macrophage MH-S cell line. The effects of sonicated protein fractions and intact M. hyopneumoniae on the gene expression of cytokines and iNOS were assessed using RT-PCR. The Annealing Control Primer (ACP)-based PCR method was used to screen differentially expressed genes. Increased transcription of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, COX-2, and iNOS mRNA was observed after exposure to the supernatant (SPT), precipitant (PPT), and intact M. hyopneumoniae protein. A time-dependent analysis of the mRNA expression revealed an upregulation after 4 h for IL-6 and iNOS and after 12 h for IL-1β and TNF-α, for both SPT and PPT; the fold change in COX-2 expression was less. A dose- and time-dependent correlation was observed in nitrite (NO) production for both protein fractions; however, there was no significant difference between the effects of the two protein fractions. In a differential gene analysis, PCR revealed differential expression for nine gene bands after 3 h of stimulation — only one gene was downregulated, while the remaining eight were upregulated. The results of this study provide insights that help improve our understanding of the mechanisms underlying the pathogenesis of and macrophage defenses against M. hyopneumoniae assault, and suggest targets for future studies on therapeutic interventions for M. hyopneumoniae infections.
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참고문헌 (23)
참고문헌 신청
Asai T / 1993 / Increased levels of tumor necrosis factor and interleukin 1 in bronchoalveolar lavage fluids from pigs infected with Mycoplasma hyopneumoniae / Vet. Immunol.
Asai T / 1994 / Detection of interleukin-6 and prostaglandin E2 in bronchoalveolar lavage fluids of pigs experimentally infected with Mycoplasma hyopneumoniae / Vet. Immunol.
Choi C / 2006 / Expression of inflammatory cytokines in pigs experimentally infected with Mycoplasma hyopneumoniae / J. Comp. Pathol 134 : 40 ~ 46
Chow JC / 1999 / Toll-like receptor-4 mediates lipopolysaccharideinduced signal transduction / J. Biol. Chem 274 : 10689 ~ 10692
Chu NR / 2000 / Immunotherapy of a human papillomavirus type 16E7-expressing tumor by administration of fusion protein comprised of Mycobacterium bovis BCG Hsp65 and HPV16E7
Asai T / 1994 / Detection of interleukin-6 and prostaglandin E2 in bronchoalveolar lavage fluids of pigs experimentally infected with Mycoplasma hyopneumoniae / Vet. Immunol.
Choi C / 2006 / Expression of inflammatory cytokines in pigs experimentally infected with Mycoplasma hyopneumoniae / J. Comp. Pathol 134 : 40 ~ 46
Chow JC / 1999 / Toll-like receptor-4 mediates lipopolysaccharideinduced signal transduction / J. Biol. Chem 274 : 10689 ~ 10692
Chu NR / 2000 / Immunotherapy of a human papillomavirus type 16E7-expressing tumor by administration of fusion protein comprised of Mycobacterium bovis BCG Hsp65 and HPV16E7
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