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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 397 - 406 (10page)
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초록· 키워드
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Background C1q and TNF related protein 1 (C1QTNF1) is known to be associated with coronary artery diseases. However, the molecular function of C1QTNF1 on the vascular smooth muscles remains to be investigated. Objective This study was therefore undertaken to investigate the effect of C1QTNF1 on gene expression of human smooth muscle cells and to reveal potential molecular mechanisms mediated by C1QTNF1. Methods Vascular smooth muscle cells were incubated with recombinant C1QTNF1 for 16 h, followed by determining any change in mRNA expressions by Affymetrix genechip. Gene ontology (GO), KEGG pathway, and protein–protein interaction (PPI) network were analyzed in differentially expressed genes. In addition, validation of microarray data was performed using quantitative real-time PCR. Results The mRNA expressions of annotated 74 genes were significantly altered after incubation with recombinant C1QTNF1; 41 genes were up-regulated and 33 down-regulated. The differentially expressed genes were enriched in biological processes and KEGG pathways associated with inflammatory responses. In the PPI network analysis, IL-6, CCL2, and ICAM1 were identified as potential key genes with relatively high degree. The cluster analysis in the PPI network identified a significant module composed of upregulated genes, such as IL-6, CCL2, NFKBIA, SOD2, and ICAM1. The quantitative real-time PCR results of potential key genes were consistent with microarray data. Conclusion The results in the present study provide insights on the effects of C1QTNF1 on gene expression of smooth muscle cells. We believe our findings will help to elucidate the molecular mechanisms regarding the functions of C1QTNF1 on smooth muscle cells in inflammatory diseases.
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참고문헌 (35)
참고문헌 신청
Aiello RJ / 1999 / Monocyte chemoattractant protein-1 accelerates atherosclerosis in apolipoprotein E-deficient mice / Arterioscler Thromb Vasc Biol 19:1518~1525
Akiyama H / 2013 / A novel adipokine C1q/TNF-related protein 1(CTRP1)regulates chondrocyte proliferation and maturation through the ERK1/2 signaling pathway / Mol Cell Endocrinol 369:63~71
Baeuerle PA / 1996 / NF-kappa B : ten years after / Cell 87:13~20
Braun M / 1999 / Cellular adhesion molecules on vascular smooth muscle cells / Cardiovasc Res 41:395~401
Cheng X / 2017 / Claudin-1 regulates pulmonary artery smooth muscle cell proliferation through the activation of ERK1/2 / Biomed Pharmacother 89:983~990
Akiyama H / 2013 / A novel adipokine C1q/TNF-related protein 1(CTRP1)regulates chondrocyte proliferation and maturation through the ERK1/2 signaling pathway / Mol Cell Endocrinol 369:63~71
Baeuerle PA / 1996 / NF-kappa B : ten years after / Cell 87:13~20
Braun M / 1999 / Cellular adhesion molecules on vascular smooth muscle cells / Cardiovasc Res 41:395~401
Cheng X / 2017 / Claudin-1 regulates pulmonary artery smooth muscle cell proliferation through the activation of ERK1/2 / Biomed Pharmacother 89:983~990
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