메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색

논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제2호
발행연도
2007.1
수록면
207 - 217 (11page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색

초록· 키워드

오류제보하기
The Saccharomyces cerevisiae KNU5377 strain,which was isolated from spoilage in nature, has the ability toconvert biomass to alcohol at high temperatures and it canresist against various streses [18, 19]. In order to understandthe defense mechanisms of the KNU5377 strain under menadione(MD) as oxidative stress, we used several techniques forstudy: peptide mas fingerprinting (PMF) by matrix-asistedspectrometry (MS) followed by two-dimensional (2D) gelelectrophoresis, liquid chromatography electrospray ionizationtandem mass spectrometry (LC-ESI-MS/MS), and surface-enhanced laser desorption ionization-time of flight (SELDI-TOF)technology. Among the 35 proteins identified by MALDI-TOFMS, 19 proteins including Sod1p, Sod2p, Tsa1p, and Ahp1pwere induced under stress condition, while 16 proteins wereaugmented under normal condition. In particular, five proteins,Sod1p, Sod2p, Ahp1p, Rib3p, Yaf9p, and Mnt1p, were inducedin only stressed cells. By LC-ESI-MS/MS analysis, 37 proteinsthe stressed cells. Among the identified proteins, 32 proteins werefound in both cells. Five proteins including Yel047cp and Met6pwere only upregulated in the normal cells, whereas 17 proteinsincluding Abp1p and Sam1p were elevated in the stressed cells.It was interesting that highly hypothetical proteins such asYnl281wp, Ygr279cp, Ypl273wp, Ykl133cp, and Ykr074wpwere only expressed in the stressed cells. SELDI-TOF analysisusing the SAX2 and WCX2 chips showed that highly multiple-specific protein patterns were reproducibly detected in rangesTherefore, induction of antioxidant proteins, hypotheticalproteins, and low molecular weight proteins were revealed bydiferent proteomic techniques. These results suggest thatcomparative analyses using proteomics might contribute toelucidate the defense mechanisms of KNU5377 under MD stress.

목차

등록된 정보가 없습니다.

참고문헌 (28)

참고문헌 신청

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0