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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Hong-Nam (Division of Life Sciences, College of Natural Sciences Chungbuk National University) Cha, Jae-Soon (Department of Agricultural Biology, College of Agriculture, Chungbuk National University) Cho, Tae-Ju (Division of Life Sciences, College of Natural Sciences Chungbuk National University) Kim, Hak-Yong (Division of Life Sciences, College of Natural Sciences Chungbuk National University)
저널정보
한국통합생물학회 Korean journal of biological sciences Korean journal of biological sciences 제7권 제3호
발행연도
2003.1
수록면
213 - 219 (7page)

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The response of plants to pathogens and wounding is dependent upon very sensitive perception mechanisms. Although genetic approaches have revealed a variety of resistance genes that activate common defense responses, defense-related proteins are not well characterized in plants. Therefore, we used a proteomic approach to determine which defense-related proteins are induced by salicylic acid (SA) and wounding in Chinese cabbage. We found that SA and wounding induce pathogenesis-related protein 1a (PR1a) at both protein and mRNA levels using proteomics and Northern blot analysis, respectively. This indicates that our proteomic approach is useful for identifying defense-related proteins. We also identified several other proteins that are induced by SA or wounding. Among the seven SA-induced proteins identified, four may be defense-related, including defense-related protein, phospholipase D (PLD), resistance protein RPS2 homolog, and L-ascorbate peroxidase. Out of the six wounding-induced proteins identified, three may be defense-related: heat shock cognate protein 70 (HSC70), polygalacturonase, and peroxidase P7. The precise functions of these proteins in plant defense responses await further study. However, identification of the defense-related proteins described in this study should allow us to better understand the mechanisms and signal transduction pathways involved in defense responses in Chinese cabbage.

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