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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제2호
- 발행연도
- 2017.1
- 수록면
- 321 - 334 (14page)
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초록· 키워드
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Process surveillance within agricultural biogas plants (BGPs) was concurrently studied by highthroughput 16S rRNA gene amplicon sequencing and an optimized quantitative microscopic fingerprinting (QMF) technique. In contrast to 16S rRNA gene amplicons, digitalized microscopy is a rapid and cost-effective method that facilitates enumeration and morphological differentiation of the most significant groups of methanogens regarding their shape and characteristic autofluorescent factor 420. Moreover, the fluorescence signal mirrors cell vitality. In this study, four different BGPs were investigated. The results indicated stable process performance in the mesophilic BGPs and in the thermophilic reactor. Bacterial subcommunity characterization revealed significant differences between the four BGPs. Most remarkably, the genera Defluviitoga and Halocella dominated the thermophilic bacterial subcommunity, whereas members of another taxon, Syntrophaceticus, were found to be abundant in the mesophilic BGP. The domain Archaea was dominated by the genus Methanoculleus in all four BGPs, followed by Methanosaeta in BGP1 and BGP3. In contrast, Methanothermobacter members were highly abundant in the thermophilic BGP4. Furthermore, a high consistency between the sequencing approach and the QMF method was shown, especially for the thermophilic BGP. The differences elucidated that using this biphasic approach for mesophilic BGPs provided novel insights regarding disaggregated single cells of Methanosarcina and Methanosaeta species. Both dominated the archaeal subcommunity and replaced coccoid Methanoculleus members belonging to the same group of Methanomicrobiales that have been frequently observed in similar BGPs. This work demonstrates that combining QMF and 16S rRNA gene amplicon sequencing is a complementary strategy to describe archaeal community structures within biogas processes.
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참고문헌 (22)
참고문헌 신청
Ali Shah F / 2014 / Microbial ecology of anaerobic digesters: the key players of anaerobiosis / Sci. World J 2014 : e183752 ~
Demirel B / 2008 / The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review / Rev. Environ. Sci. Biotechnol 7 :
Eikmeyer FG / 2013 / Detailed analysis of metagenome datasets obtained from biogas-producing microbial communities residing in biogas reactors does not indicate the presence
Hansen KH / 1998 / Anaerobic digestion of swine manure: inhibition by ammonia / Water Res 32 : 5 ~ 12
Jaenicke S / 2011 / Comparative and joint analysis of two metagenomic datasets from a biogas fermenter obtained by 454-pyrosequencing / PLoS One 6 : e14519 ~
Demirel B / 2008 / The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review / Rev. Environ. Sci. Biotechnol 7 :
Eikmeyer FG / 2013 / Detailed analysis of metagenome datasets obtained from biogas-producing microbial communities residing in biogas reactors does not indicate the presence
Hansen KH / 1998 / Anaerobic digestion of swine manure: inhibition by ammonia / Water Res 32 : 5 ~ 12
Jaenicke S / 2011 / Comparative and joint analysis of two metagenomic datasets from a biogas fermenter obtained by 454-pyrosequencing / PLoS One 6 : e14519 ~
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