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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제12호
- 발행연도
- 2010.1
- 수록면
- 1,756 - 1,763 (8page)
이용수
초록· 키워드
오류제보하기
Pasteurella multocida serogroup D strain, which produces P. multocida toxin (PMT), is a widespread and harmful pathogen of respiratory diseases such as pneumonia and progressive atrophic rhinitis (PAR) in swine. Vaccination has been considered the most desirable and effective approach for controlling the diseases caused by toxigenic P.
multocida. To investigate the antigenicity and immunogenicity of partial fragments of recombinant PMT, recombinant proteins of the N-terminal (PMT-A), middle (PMT-B), Cterminal (PMT-C), and middle-C-terminal (PMT2.3) regions of PMT were successfully produced in an Escherichia coli expression system. The molecular masses of PMT-A,PMT-B, PMT-C, and PMT2.3 were ca. 53, 55, 35, and 84 kDa, respectively, purified by nickel-nitrilotriacetic acid (Ni-NTA) affinity column chromatography. All the recombinant proteins except for PMT-A showed immune responses to antisera obtained from a swine showing symptoms of PAR. Moreover, high titers of PMT-specific antibodies were raised from mice immunized with each of the recombinant proteins; however, the immunoreactivities of the antibodies to authentic PMT and heat-inactivated whole bacteria were different, respectively. In the protection study, the highest protection against homologous challenge was shown in the case of PMT2.3; relatively poor protections occurred for the other PMT fragments.
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참고문헌 (23)
참고문헌 신청
Adler, B / 1999 / Candidate vaccine antigens and genes in Pasteurella multocida / J. Biotechnol 73 : 83 ~ 90
Bagley, K. C / 2005 / Pasteurella multocida toxin activates human monocyte-derived and murine bone marrow-derived dendritic cells in vitro but suppresses antibody production
Baldwin, M. R / 2004 / Identification and characterization of the Pasteurella multocida toxin translocation domain / Mol. Microbiol 54 : 239 ~ 250
Brown, W. F / 1964 / Variance estimation in the Reed-Muench fifty per cent end-point determination / Am. J. Hyg 79 : 37 ~ 46
Busch, C / 2001 / Biological activity of a C-terminal fragment of Pasteurella multocida toxin / Infect. Immun 69 : 3628 ~ 3634
Bagley, K. C / 2005 / Pasteurella multocida toxin activates human monocyte-derived and murine bone marrow-derived dendritic cells in vitro but suppresses antibody production
Baldwin, M. R / 2004 / Identification and characterization of the Pasteurella multocida toxin translocation domain / Mol. Microbiol 54 : 239 ~ 250
Brown, W. F / 1964 / Variance estimation in the Reed-Muench fifty per cent end-point determination / Am. J. Hyg 79 : 37 ~ 46
Busch, C / 2001 / Biological activity of a C-terminal fragment of Pasteurella multocida toxin / Infect. Immun 69 : 3628 ~ 3634
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