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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제12호
- 발행연도
- 2008.1
- 수록면
- 1,945 - 1,952 (8page)
이용수
초록· 키워드
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Sialylation, the attachment of sialic acid residues to a
protein, can affect the biological activity and in vivo
circulatory half-life of glycoproteins. Human α2,3-
sialyltransferase (α2,3-ST) and β1,4-galactosyltransferase
(β1,4-GT) are responsible for terminal sialylation and
galactosylation, respectively. Enhanced sialylation of
human erythropoietin (EPO) by the expression of α2,3-ST
and β1,4-GT was achieved using recombinant Chinese
hamster ovary (CHO) cells (EC1). The sialic acid content
and sialylation of N-glycans were evaluated by HPLC.
When α2,3-ST was expressed in CHO cells (EC1-ST2),
the sialic acid content (moles of sialic acid/mole of EPO)
increased from 6.7 to 7.5. In addition, the amount of trisialylated
glycans increased from 17.3% to 26.1%. When
α2,3-ST and β1,4-GT were coexpressed in CHO cells
(EC1-GTST15), the degree of sialylation was greater than
that in EC1-ST2 cells. In the case of EC1-GTST15 cells,
the sialic acid content increased to 8.2 and the proportion
of trisialylated glycans was markedly increased from
17.3% to 35.5%. Interestingly, the amount of asialoglycans
decreased only in the case of GTST15 cells (21.4%
to 14.2%). These results show that coexpression of α2,3-
ST and β1,4-GT is more effective than the expression of
α2,3-ST alone. Coexpression of α2,3-ST and β1,4-GT did
not affect CHO cell growth and metabolism or EPO
production. Thus, coexpression of α2,3-ST and β1,4-GT
may be beneficial for producing therapeutic glycoproteins
with enhanced sialylation in CHO cells.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Ailor, E. / 2000 / N-Glycan patterns of human transferrin produced in Trichoplusia ni insect cells:Effects of mammalian galactosyltransferase.Glycobiology 10:837-847 / Glycob
Anumula, K. R. / 1995 / Rapid quantitative determination of sialic acids in glycoproteins by high-performance liquid chromatography with a sensitive fluorescence detection /
Chang, K. H. / 1999 / N-Acetylcysteine increases the biosynthesis of recombinant EPO in apoptotic Chinese hamster ovary cells / Free Radic. Res 30 : 85 ~ 91
Choi, O. / 2003 / N-Glycan structures of human transferrin produced by Lymantria dispar (gypsy moth) cells using the LdMNPV expression system / Glycobiology 13 : 539 ~ 548
Chung, B. S. / 2001 / Effect of sodium butyrate on glycosylation of recombinant erythropoietin. J. Microbiol / Biotechnol 11 : 1087 ~ 1092
Anumula, K. R. / 1995 / Rapid quantitative determination of sialic acids in glycoproteins by high-performance liquid chromatography with a sensitive fluorescence detection /
Chang, K. H. / 1999 / N-Acetylcysteine increases the biosynthesis of recombinant EPO in apoptotic Chinese hamster ovary cells / Free Radic. Res 30 : 85 ~ 91
Choi, O. / 2003 / N-Glycan structures of human transferrin produced by Lymantria dispar (gypsy moth) cells using the LdMNPV expression system / Glycobiology 13 : 539 ~ 548
Chung, B. S. / 2001 / Effect of sodium butyrate on glycosylation of recombinant erythropoietin. J. Microbiol / Biotechnol 11 : 1087 ~ 1092
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