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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제5호
- 발행연도
- 2008.1
- 수록면
- 845 - 851 (7page)
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TolC is an outer membrane porin protein and an essential component of drug efflux and type-I secretion systems in Gram-negative bacteria. TolC comprises a periplasmic α- helical barrel domain and a membrane-embedded β-barrel domain. TdeA, a functional and structural homolog of TolC, is required for toxin and drug export in the pathogenic oral bacterium Actinobacillus actinomycetemcomitans. Here, we report the expression of the periplasmic domain of TdeA as a soluble protein by substitution of the membraneembedded domain with short linkers, which enabled us to purify the protein in the absence of detergent. We confirmed the structural integrity of the TdeA periplasmic domain by size-exclusion chromatography, circular dichroism spectroscopy, and electron microscopy, which together showed that the periplasmic domain of the TolC protein family fold correctly on its own. We further demonstrated that the periplasmic domain of TdeA interacts with peptidoglycans of the bacterial cell wall, which supports the idea that completely folded TolC family proteins traverse the peptidoglycan layer to interact with inner membrane transporters.
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참고문헌 (15)
참고문헌 신청
CHOI, JONG HYUN / 2005 / Display of Proteins on the Surface of Escherichia coli by C-Terminal Deletion Fusion to the Salmonella typhimurium OmpC / Journal of Microbiology and
Crosby, J. A. / 2007 / TdeA, a TolC-like protein required for toxin and drug export in Aggregatibacter (Actinobacillus) actinomycetemcomitans / Gene 388 : 83 ~ 92
DeLano, W. / 2002 / The PyMOL User’s Manual / DeLano Scientific
Dmitriev, B. / 2005 / Towards a comprehensive view of the bacterial cell wall / Trends Microbiol 13 : 569 ~ 574
Koronakis, V. / 1997 / Structure of TolC, the outer membrane component of the bacterial type I efflux system, derived from two-dimensional crystals. Mol / Microbiol 23 : 617
Crosby, J. A. / 2007 / TdeA, a TolC-like protein required for toxin and drug export in Aggregatibacter (Actinobacillus) actinomycetemcomitans / Gene 388 : 83 ~ 92
DeLano, W. / 2002 / The PyMOL User’s Manual / DeLano Scientific
Dmitriev, B. / 2005 / Towards a comprehensive view of the bacterial cell wall / Trends Microbiol 13 : 569 ~ 574
Koronakis, V. / 1997 / Structure of TolC, the outer membrane component of the bacterial type I efflux system, derived from two-dimensional crystals. Mol / Microbiol 23 : 617
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