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논문 기본 정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제4호
- 발행연도
- 2008.1
- 수록면
- 695 - 703 (9page)
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초록· 키워드
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A locally isolated thermophile, Geobacillus sp. SAB-40,
producing thermostable extracellular amylase constitutively
and an induced intracellular β-galactosidase was
characterized and identified based on 16S rRNA sequencing.
A phylogenetic analysis then revealed its closeness to
Geobacillus stearothermophilus. To evaluate the effect of
the culture conditions on the coproduction of both enzymes
by G. stearothermophilus SAB-40, a Plackett-Burman
fractional factorial design was applied to determine the
impact of twenty variables. Among the tested variables,
CaCl₂, the incubation time, MgSO₄·7H₂O, and tryptone
were found to be the most significant for encouraging
amylase production. Lactose was found to promote β-
galactosidase production, whereas starch had a significantly
negative effect on lactase production. Based on a statistical
analysis, a preoptimized medium attained the maximum
production of amylase and β-galactosidase at 23.29 U/ml/
min and 12,958 U/mg biomass, respectively, which was 3-
and 2-fold higher than the yield of amylase and lactase
obtained with the basal medium, respectively.
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