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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제4호
- 발행연도
- 2008.1
- 수록면
- 624 - 630 (7page)
이용수
초록· 키워드
오류제보하기
Terminal restriction fragment length polymorphism (TRFLP) is a method that has been frequently used to survey the microbial diversity of environmental samples and to monitor changes in microbial communities. T-RFLP is a highly sensitive and reproducible procedure that combines a PCR with a labeled primer, restriction digestion of the amplified DNA, and separation of the terminal restriction fragment (T-RF). The reliable identification of T-RF requires the information of nucleotide sequences as well as the size of T-RF. However, it is difficult to obtain the information of nucleotide sequences because the T-RFs are fragmented and lack a priming site of 3'-end for efficient cloning and sequence analysis. Here, we improved on the T-RFLP method in order to analyze the nucleotide sequences of the distinct TRFs.
The first method is to selectively amplify the portion of T-RF ligated with specific oligonucleotide adapters. In the second method, the termini of T-RFs were tailed with deoxynucleotides using terminal deoxynucleotidyl transferase (TdT) and amplified by a second round of PCR. The major T-RFs generated from reference strains and from T-RFLP profiles of activated sludge samples were efficiently isolated and identified by using two modified T-RFLP methods.
These methods are less time consuming and labor-intensive when compared with other methods. The T-RFLP method using TdT has the advantages of being a simple process and having no limit of restriction enzymes. Our results suggest that these methods could be useful tools for the taxonomic interpretation of T-RFs.
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참고문헌 (19)
참고문헌 신청
AHN, JAE-HYUNG / 2006 / Improvement of PCR Amplification Bias for Community Structure Analysis of Soil Bacteria by Denaturing Gradient Gel Electrophoresis / Journal of Microb
Blackwood, C. B. / 2007 / Evaluating the physical capture method of terminal restriction fragment length polymorphism for comparison of soil microbial communities. Soil Biol
Eldridge, M. L. / 2007 / The response of bacterial groups to changes in available iron in the Eastern subtropical Pacific Ocean. J. Exp. Mar. Biol / Ecol 348 : 11 ~ 22
Horz, H. P. / 2001 / Detection of methanotroph diversity on roots of submerged rice plants by molecular retrieval of pmoA, mmoX, mxaF, and 16S rRNA and ribosomal DNA, includi
Hugenholtz, P. / 1998 / Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity / Bacteriol 180 : 4765 ~ 4774
Blackwood, C. B. / 2007 / Evaluating the physical capture method of terminal restriction fragment length polymorphism for comparison of soil microbial communities. Soil Biol
Eldridge, M. L. / 2007 / The response of bacterial groups to changes in available iron in the Eastern subtropical Pacific Ocean. J. Exp. Mar. Biol / Ecol 348 : 11 ~ 22
Horz, H. P. / 2001 / Detection of methanotroph diversity on roots of submerged rice plants by molecular retrieval of pmoA, mmoX, mxaF, and 16S rRNA and ribosomal DNA, includi
Hugenholtz, P. / 1998 / Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity / Bacteriol 180 : 4765 ~ 4774
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