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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제10호
- 발행연도
- 2007.1
- 수록면
- 1,727 - 1,732 (6page)
이용수
초록· 키워드
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Phenylacetic acid (PA) is produced by manybacteria as antifungal agent and also appears to be anenvironmentaly toxic chemical. The object of this study wasto detect PAA using Pseudomonas putida harboring a reporterplasmid that has a PA-inducible promoter fused to a greenfluorescent protein (GFP) gene. Pseudomonas putida KT2440was used to construct a green fluorescent protein-basedreporter fusion using the paaApresence of PA. The reporter strain exhibited a high level ofgfp expression in minimal medium containing PA; however,the level of GFP expresion diminished when glucose wasaded to the medium, whereas other carbon sources, such assucinate and pyruvate, showed no catabolic repression.Interestingly, overexpression of a paaF gene encoding PAA-CoA ligase minimized catabolic repression. The reporterstrain could also sucesfully detect PA produced by othera useful tool for detecting bacteria producing PAA.
#Biosensor
#GFP
#biodegradation
#catabolic repression
#transcriptional regulationPhenylacetic acid
#PAA) is an important organic chemicalmaterial that is widely used in the field of medicine
#asa pesticide
#and as an aromatizer [32]. PA is a metabolic
#Biosensor
#GFP
#biodegradation
#catabolic repression
#transcriptional regulationPhenylacetic acid
#PAA) is an important organic chemicalmaterial that is widely used in the field of medicine
#asa pesticide
#and as an aromatizer [32]. PA is a metabolic
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Ausubel, F. M / 1999 / Current Protocolsin Molecular Biology
B / / and J. M. Luengo. 20. Phenylacetyl-coenzyme A is the true inducer of the phenylacetic acidcatabolism pathway in Pseudomonas putida U. Appl.Environ. Microbiol. 66 : 4575
Bartolom-Martn, D., E. Martnez-Garca, V. Mascaraque,J. Rubio, J. Perera, and S. Alonso / 2004 / Characterization of asecond functional gene cluster for the catabolism of phen
Burlage, R. S / 1997 / Emerging technologies: Bioreporters,biosensors and microprobes : 115 ~ 123
Collier, D. N / 1996 / Catabolite repression control in Pseud 147 : 551 ~ 561
B / / and J. M. Luengo. 20. Phenylacetyl-coenzyme A is the true inducer of the phenylacetic acidcatabolism pathway in Pseudomonas putida U. Appl.Environ. Microbiol. 66 : 4575
Bartolom-Martn, D., E. Martnez-Garca, V. Mascaraque,J. Rubio, J. Perera, and S. Alonso / 2004 / Characterization of asecond functional gene cluster for the catabolism of phen
Burlage, R. S / 1997 / Emerging technologies: Bioreporters,biosensors and microprobes : 115 ~ 123
Collier, D. N / 1996 / Catabolite repression control in Pseud 147 : 551 ~ 561
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