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자료유형
학술저널
저자정보
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한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제3호
발행연도
2006.1
수록면
423 - 430 (8page)

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초록· 키워드

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A total of 367 isolates of Phytophthora infestanswas colected from the leaf samples of late blight diseasefrom five provinces in Korea over the three growing seasonsof 2002-204. Of the 367 isolates, 337 isolates were of theA1 mating type, and 30 isolates were of A2 mating type,showing that the majority was A1 mating type. Profiles of Gpiand Pep defined four allozyme genotypes among the total of367 isolates. All four allozyme genotypes could be distinguishedon the basis of Gpihomozygous at the Pep locus (100/100). The mitochondrialDNA haplotype of al isolates were the IIa haplotype.Amplification of the genomic DNAs extracted from eightisolates of each mating type by polymerase chain reactionwith the selected primer (OPC-5 primer) produced a total of20 DNA bands, of which 11 bands were polymorphic.According to the RAPD analysis using the OPC-5 primer, 106isolates including two standard isolates were separated into 8were not correlated with the alozyme genotypes and theisolated locations. All of the eight RAPD groups wereidentified in Gangwon-do, suggesting that Gangwon-do is thecenter of origin of the P. infestans in Korea. A 600-bp DNAband generated with the OPC-5 primer was specific to A1mating type isolates, but not detected with A2 mating type,showing that the specific PCR primer can distinguish differentmating types in P. infestans.

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