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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제5호
- 발행연도
- 2005.1
- 수록면
- 952 - 964 (13page)
이용수
초록· 키워드
오류제보하기
The diesel-degrading activities of biofilms sampledfrom petroleum-contaminated groundwaters in urban subwaydrainage systems were examined in liquid cultures, and themicrobial populations of the biofilms were characterized bydenaturing gel gradient electrophoresis (DGE) and 16SrDNA sequence analysis. Biofilm samples derived from twosites (19 K and 20 K) at subway Station N and Station I coulddegrade around 80% of applied diesel within 20 and 40 days,respectively, at 15oC, and these results were strongly correlatedwith the growth paterns of the biofilms. The closest phylogeneticneighbor of a dominant component in the 19 K biofilm wasThiothrix fructosivorans strain Q (100% similarity). Fourdominant strains in the 20 K biofilm were closely related toThiothrix fructosivorans strain Q (100% similarity), Thiothrixsp. CC-5 (100% similarity), Sphaerotilus sp. IF14 (99%similarity), and Cytophaga-Flexibacter-Bacterioides (CFB)group bacterium RW262 (98% similarity). Three dominantmembers in the Station I biofilms were very similar touncultured Cytophagales clone CRE-PA82 (91% similarity),Pseudomonas sp. WDL5 (97% similarity), and unculturedCFB group bacterium LCK-64 (94% similarity). The microbialcomponents of the biofilms differed depending on thesampling site. This is the first report on the isolation of cloneshighly similar to Thiothrix fructosivorans and Thiothrix sp.from biofilms in petroleum-polluted groundwaters, and thefirst evidence that these organisms may play major roles inpetroleum degradation and/or biofilm-development.
목차
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참고문헌 (22)
참고문헌 신청
Abed, R. M. M / 2002 / Microbial diversity of a heavily polluted microbial mat and its community changes following degradation of petroleum compounds / Appl. Environ. Microbi
Alcantara, S / 2004 / Hydrogen sulfide oxidation by a microbial consortium in a recirculation reactor system: Sulfur formation under oxygen limitation and removal of phenols
Alfreider, A / 2002 / Microbial diversity in an in situ reactor system treating monochlorobenzene contaminated groundwater as revealed by 16S ribosomal DNA analysis / Syst. A
An, Y. J / 2004 / Microbial characterization of toluene-degrading denitrifying consortia obtained from terrestrial and marine ecosystems / Appl. Microbiol. Biotechnol. 65 : 6
BAEK, KYUNG-HWA / 2004 / Effects of Soil Types on the Biodegradation ofCrude Oil by Nocardia sp. H17-1 / Journal of Microbiology and Biotechnology 14 (5) : 901 ~ 905
Alcantara, S / 2004 / Hydrogen sulfide oxidation by a microbial consortium in a recirculation reactor system: Sulfur formation under oxygen limitation and removal of phenols
Alfreider, A / 2002 / Microbial diversity in an in situ reactor system treating monochlorobenzene contaminated groundwater as revealed by 16S ribosomal DNA analysis / Syst. A
An, Y. J / 2004 / Microbial characterization of toluene-degrading denitrifying consortia obtained from terrestrial and marine ecosystems / Appl. Microbiol. Biotechnol. 65 : 6
BAEK, KYUNG-HWA / 2004 / Effects of Soil Types on the Biodegradation ofCrude Oil by Nocardia sp. H17-1 / Journal of Microbiology and Biotechnology 14 (5) : 901 ~ 905
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