지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제14권 제2호
- 발행연도
- 2004.1
- 수록면
- 231 - 236 (6page)
이용수
초록· 키워드
오류제보하기
The inexpensive large-scale production of purePGA (Penicillin G Acylase) has been a commercial goal.PGA has been used as a model enzyme in the development ofsimple one-step purification methods. In this study, thepurification of poly-His tagged PGA protein secreted into theperiplasmic space was carried out by using immobilizedmetal-ion affinity chromatography (IMAC). The PGA genewas obtained from E. coli ATCC 11105. Codons encodinghistidines were fused at the C-terminus of the PGA gene byPCR. E. coli JM109 harboring pPGA-HIS6 vector producedactive his-tagged acylases in the presence of lac promoterduring cultivation at 26oC. The maximum specific activity ofthe acylase purified by using one-step chromatography afterosmotic shock was 38.5 U/mg and was recovered with theyield of 70%. Both 23 kDa (a) and 62 kDa (b) subunits wererecovered by using IMAC with just C-terminus tagging of theb subunit. The purification of the periplasmic fraction byosmotic shock and that of purified acylase was increased by2.6-fold and 19-fold, respectively, compared to the crudeextract.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
/ / Identification of catalyticallyessential amino acid residues of penicillin G acylaseobtained from a mutant of Escherichia coli ATCC 11105 : 861 ~ 867
/ 1972 / The isolation and kinetics of penicillin amidase fromEscherichia coli : 250 ~ 276 256
/ 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding : 248 ~ 254
/ 1986 / Penicillin G acylase from E : 5713 ~ 5727
/ 1987 / Complete nucleotide sequence of thepenicillin G acylase gene and the flanking regions and itsexpression in Escherichia coli : 87 ~ 97
/ 1972 / The isolation and kinetics of penicillin amidase fromEscherichia coli : 250 ~ 276 256
/ 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding : 248 ~ 254
/ 1986 / Penicillin G acylase from E : 5713 ~ 5727
/ 1987 / Complete nucleotide sequence of thepenicillin G acylase gene and the flanking regions and itsexpression in Escherichia coli : 87 ~ 97
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Penicillin G acylase 遺傳子의 構造와 發現機作에 關한 硏究 : I. E. coli ATCC 11105의 penicillin G acylase 遺傳子의 Cloning
미생물학회지
1983 .06
-
Bacillus megaterium에서 발견된 Penicillin G Acylase 유전자의 염기서열과 그 효소의 특성
미생물학회지
1994 .06
-
Cephalosporin C Acylase 생산균주의 선발 및 특성
한국동물학회 학술대회
1994 .01
-
Penicillin G Amidase생산을 위한 재조합 대장균의 유가배양에 관한 연구
한국미생물·생명공학회지
2008 .01
-
Covalent Immobilization of Penicillin G Acylase onto Fe3O4@Chitosan Magnetic Nanoparticles
Journal of Microbiology and Biotechnology
2016 .01
-
Glutaryl 7-Aminodeacetoxycephalosporanic Acid Acylase 생산균의 분리 및 동정
미생물학회지
1994 .06
-
Biosynthesis of Cephalexin in PEG400-Ammonium Sulfate and PEG400-Magnesium Sulfate Aqueous Two-Phase Systems
Journal of Microbiology and Biotechnology
2004 .01
-
Escherichia coli GroEL was Induced by the Expression of the Cloned Bacillus megaterium ATCC14945 Penicillin G Acylase Gene
미생물학회지
1992 .12
-
Isolation of Quorum Quenching Pseudomonas sp. 1A1 from a Membrane Bioreactor for Wastewater Treatment, and Cloning of Three AHL-Acylase Genes from the Isolate
한국미생물학회 학술대회논문집
2013 .05
-
An Efficient Method for the Release of Recombinant Penicillin G Amidase from the Escherichia coli Periplasm
생명과학회지
2017 .10
-
Immobilization and Performance of Penicillin G Acylase on Magnetic Ni0.7Co0.3Fe2O4@SiO2-CHO Nanocomposites
Journal of Microbiology and Biotechnology
2019 .01
-
Effects of Penicillin G on Morphology and Certain Physiological Parameters of Lactobacillus acidophilus ATCC 4356
Journal of Microbiology and Biotechnology
2011 .01
-
Isolation of Novel Pseudomonas diminuta KAC-1 Strain Producing Glutaryl 7-Aminocephalosporanic Acid Acylase
The Journal of Microbiology
1999 .12
-
Purification and Characterization of NADPH-Dependent Cr(Ⅵ) Reductase from Escherichia coli ATCC 33456
The Journal of Microbiology
2005 .02
-
Purification and Partial Characterization of the 25 kDa Protein Antigen of Mycobacterium tuberculosis
The Journal of the Korean Society for Microbiology
1995 .04
-
Penicillium chrysogenum에 의한 ${\beta}-Lactamase$ 생성(生成)
한국균학회지
1980 .01
-
Escherichia coli K12의 막단백질 형성과 페니실린 민감성에 대한 산소의 능동적 역할
미생물학회지
1986 .09
-
결핵균 30-kDa와 32-kDa 단백항원의 정제와 Lymphokine에 의한 사람 단핵세포의 활성화
The Journal of the Korean Society for Microbiology
1993 .04
-
Expression and Purification of Mouse Sulfated Glycoprotein-2 in E. coli.
한국동물학회 학술대회
1994 .01
-
Crystal Structures of Bifunctional Penicillin-Binding Protein 4 from Listeria monocytogenes
한국미생물학회 학술대회논문집
2014 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0