지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제1호
- 발행연도
- 2016.1
- 수록면
- 80 - 88 (9page)
이용수
초록· 키워드
오류제보하기
The enzyme-catalyzed Henry reaction was realized using deep eutectic solvents (DESs) as a reaction medium. The lipase from Aspergillus niger (lipase AS) showed excellent catalytic activity toward the substrates aromatic aldehydes and nitromethane in choline chloride:glycerol at a molar ratio of 1:2. Addition of 30 vol% water to DES further improved the lipase activity and inhibited DES-catalyzed transformation. A final yield of 92.2% for the lipase AS-catalyzed Henry reaction was achieved under optimized reaction conditions in only 4 h. In addition, the lipase AS activity was improved by approximately 3-fold in a DES–water mixture compared with that in pure water, which produced a final yield of only 33.4%. Structural studies with fluorescence spectroscopy showed that the established strong hydrogen bonds between DES and water may be the main driving force that affects the spatial conformation of the enzyme, leading to a change in lipase activity. The methodology was also extended to the aza-Henry reaction, which easily occurred in contrast to that in pure water. The enantioselectivity of both Henry and aza-Henry reactions was not found. However, the results are still remarkable, as we report the first use of DES as a reaction medium in a lipase-catalyzed Henry reaction.
목차
등록된 정보가 없습니다.
참고문헌 (32)
참고문헌 신청
Abbott AP / 2004 / Deep eutectic solvents formed between choline chloride and carboxylic acids: versatile alternatives to ionic liquids / J. Am. Chem. Soc 126:9142~9147
Borah JC / 2004 / A highly efficient synthesis of the C-13 side-chain of taxol using Shibasaki's asymmetric Henry reaction / Tetrahedron Lett 45:3689~3691
Bunnage ME / 1994 / An expeditious asymmetric-synthesis of allophenylnorstatine / Tetrahedron 50:3975~3986
Busto E / 2011 / Protein-mediated nitroaldol addition in aqueous media. Catalytic promiscuity or unspecific catalysis / Org. Process Res. Dev 15:236~240
Busto E / 2010 / Hydrolases:catalytically promiscuous enzymes for non-conventional reactions in organic synthesis / Chem. Soc. Rev 39:4504~4523
Borah JC / 2004 / A highly efficient synthesis of the C-13 side-chain of taxol using Shibasaki's asymmetric Henry reaction / Tetrahedron Lett 45:3689~3691
Bunnage ME / 1994 / An expeditious asymmetric-synthesis of allophenylnorstatine / Tetrahedron 50:3975~3986
Busto E / 2011 / Protein-mediated nitroaldol addition in aqueous media. Catalytic promiscuity or unspecific catalysis / Org. Process Res. Dev 15:236~240
Busto E / 2010 / Hydrolases:catalytically promiscuous enzymes for non-conventional reactions in organic synthesis / Chem. Soc. Rev 39:4504~4523
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Effect of microwave irradiation on lipase-catalyzed reactions in ionic liquids
분석과학
2017 .06
-
Lipase-producing Filamentous Fungi from Non-dairy Creamer Industrial Waste
한국미생물·생명공학회지
2020 .01
-
Optimization of Medium for Lipase Production from Zygosaccharomyces mellis SG1.2 Using Statistical Experiment Design
한국미생물·생명공학회지
2021 .09
-
Potential Yeast from Indonesian Wild Forest Honey Showing Ability to Produce Lipase for Lipid Transesterification
한국미생물·생명공학회지
2019 .01
-
Isolation and Characterization of a Novel Bacterium Burkholderia gladioli Bsp-1 Producing Alkaline Lipase
Journal of Microbiology and Biotechnology
2019 .01
-
The effects of choline- and betaine- based natural deep eutectic solvents on the structure, stability and activity of lysozyme
한국분석과학회 학술대회
2019 .11
-
Mechanism study of in vitro cell lines cytotoxicity of deep eutectic solvents
한국분석과학회 학술대회
2022 .05
-
Gene Cloning, High-Level Expression, and Characterization of an Alkaline and Thermostable Lipase from Trichosporon coremiiforme V3
Journal of Microbiology and Biotechnology
2015 .01
-
유기용매 내성 리파아제와 그 이용가능성
생명과학회지
2017 .11
-
2021~2022년 서울 도심에서 측정된 HONO의 변화 특성
한국대기환경학회지(국문)
2023 .06
-
Extraction of Isoflavones and Phenolic acids from Tofu by Deep Eutectic Solvent(DES)
한국분석과학회 학술대회
2019 .05
-
Deep eutectic solvents as multi-functioning media for catechin extraction and storage
한국분석과학회 학술대회
2017 .05
-
Purification and Characterization of an Alkali-Thermostable Lipase from Thermophilic Anoxybacillus flavithermus HBB 134
Journal of Microbiology and Biotechnology
2016 .01
-
Increased mRNA Stability and Expression Level of Croceibacter atlanticus Lipase Gene Developed through Molecular Evolution Process
Journal of Microbiology and Biotechnology
2021 .01
-
Deep eutectic solvents as an efficient medium for one-step sample preparation for the chemical characterization of aromatic herbs
한국분석과학회 학술대회
2017 .11
-
Enhanced Structural Flexibility of an Organic Solvent-Tolerant Lipase by Dimethyl Sulfoxide and Ethanol
한국미생물학회 학술대회논문집
2015 .04
-
Toxicity assessment based on non-targeted metabolomics study of natural deep eutectic solvents in in vivo mice
한국분석과학회 학술대회
2020 .06
-
Toxicity assessment based on non-targeted metabolomics study of natural deep eutectic solvents in in vivo mice
한국분석과학회 학술대회
2020 .06
-
Nitrogen-doped Molybdenum Disulfide to Catalyze Hydrogen and Oxygen Evolution Reactions
새물리
2022 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0