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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제1호
- 발행연도
- 2016.1
- 수록면
- 38 - 43 (6page)
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초록· 키워드
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Novel and specific recognition elements are of central importance in the development of a pathogen detection method. Here, we describe a simple method for identifying the cell-wall binding domain (CBD) from a sequenced bacterial genome employing homology search for phage lysin genes. A putative CBD (CPF369_CBD) was identified from a genome of Clostridium perfringens type strain ATCC 13124, and its function was studied with the CBDGFP fusion protein recombinantly expressed in Escherichia coli. Fluorescence microscopy showed the specific binding of the fusion protein to C. perfringens cells, which demonstrates the potential of this method for the identification of novel bioprobes for specific detection of pathogenic bacteria.
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참고문헌 (27)
참고문헌 신청
Beres SB / 2002 / Genome sequence of a serotype M3 strain of group A Streptococcus: phage-encoded toxins, the highvirulence phenotype, and clone emergence / Proc. Natl. Acad. Sci. USA 99:10078~10083
Brussow H / 2004 / Phages and the evolution of bacterial pathogens: from genomic rearrangements to lysogenic conversion / Microbiol. Mol. Biol. Rev 68:560~602
Canchaya C / 2003 / Phage as agents of lateral gene transfer / Curr. Opin. Microbiol 6:417~424
DeLano WL / 2002 / Unraveling hot spots in binding interfaces:progress and challenges / Curr. Opin. Struct. Biol 12:14~20
Ganguly J / 2013 / The secondary cell wall polysaccharide of Bacillus anthracis provides the specific binding ligand for the C-terminal cell wall-binding domain of two phage endolysins, PlyL and PlyG / Glycobiology 23:820~832
Brussow H / 2004 / Phages and the evolution of bacterial pathogens: from genomic rearrangements to lysogenic conversion / Microbiol. Mol. Biol. Rev 68:560~602
Canchaya C / 2003 / Phage as agents of lateral gene transfer / Curr. Opin. Microbiol 6:417~424
DeLano WL / 2002 / Unraveling hot spots in binding interfaces:progress and challenges / Curr. Opin. Struct. Biol 12:14~20
Ganguly J / 2013 / The secondary cell wall polysaccharide of Bacillus anthracis provides the specific binding ligand for the C-terminal cell wall-binding domain of two phage endolysins, PlyL and PlyG / Glycobiology 23:820~832
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