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The microbial activity and bacterial comunity(FC), zinc-complexed cyanide (ZC), or nickel-complexed cyanide(NC), were studied. The thre reactors (designated as re-FC,re-ZC, and re-NC) were operated for 50 days with a stepwisedecrease of hydraulic retention time. In the re-FC and re-ZCreactors, FC or ZC was almost completely removed, whereasaproximately 80-87% of NC was removed in re-NC. Thisresult might be attributed to the high toxicity of nickel releasedafter degradation of NC. In the batch test, the sludges takenfrom re-FC and re-ZC completely degraded FC, ZC, and NC,re-FC and re-ZC showed similar properties in regard to cyanidedegradation, denaturing gradient gel electrophoresis (DGGE)analysis of the 16S rRNA gene of the bacterial comunitiesin the three reactors showed that bacterial comunity wasspecifically acclimated to each reactor. We found several bacterialsequences in DGGE bands that showed high similarity toknown cyanide-degrading bacteria such as Klebsiella spp.,Acidovorax spp., and Achromobacter xylosoxidansforming microorganism might also be one of the majormicroorganisms, since many sequences related to Zoogloea,Microbacterium, and phylum TM7 were detected in all thereactors.

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