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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 244 - 252 (9page)
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Protein disulfide isomerase A3 (PDIA3) is a major member of the protein disulfide isomerase (PDI) family. PDI proteins commonly reside in the endoplasmic reticulum and mediate important thiol-disulfide interchanges during post-translational protein folding. Unlike other PDI family members, PDIA3 is ubiquitous in various organ systems. However, its physiological activity varies in other tissues. PDIA3 has been associated with cancer, airway inflammation, neurodegenerative diseases, and metabolic diseases. However, the mechanisms of the association of PDIA3 with these pathological conditions remain unclear. Recombinant PDIA3 (rPDIA3) is needed to clarify the interactions between PDIA3 and certain physiological phenomena. In the present study, we aimed to produce highly purified rPDIA3 for use in pathological experiments. We expressed rPDIA3 with a histidine-enriched elongated peptide tag in Escherichia coli and obtained rPDIA3 at 97.8% purity using consecutive His-tag and reverse-phase chromatography. Elongated peptide tags screened from artificially designated library had dual functions for protein expression and simple purification.
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참고문헌 (30)
참고문헌 신청
Freedman RB / 1994 / Protein disulphide isomerase: building bridges in protein folding / Trends Biochem. Sci. 19:331~336
Galligan JJ / 2012 / The human protein disulfide isomerase gene family / Hum. Genomics. 6:6
Hatahet F / 2009 / Protein disulfide isomerase: a critical evaluation of its function in disulfide bond formation / Antioxid. Redox Signal. 11:2807~2850
Raykhel I / 2007 / A molecular specificity code for the three mammalian KDEL receptors / J. Cell Biol. 179:1193~1204
Choe MH / 2015 / ERp57 modulates STAT3 activity in radioresistant laryngeal cancer cells and serves as a prognostic marker for laryngeal cancer / Oncotarget 6:2654~2666
Galligan JJ / 2012 / The human protein disulfide isomerase gene family / Hum. Genomics. 6:6
Hatahet F / 2009 / Protein disulfide isomerase: a critical evaluation of its function in disulfide bond formation / Antioxid. Redox Signal. 11:2807~2850
Raykhel I / 2007 / A molecular specificity code for the three mammalian KDEL receptors / J. Cell Biol. 179:1193~1204
Choe MH / 2015 / ERp57 modulates STAT3 activity in radioresistant laryngeal cancer cells and serves as a prognostic marker for laryngeal cancer / Oncotarget 6:2654~2666
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