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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제2호
- 발행연도
- 2018.1
- 수록면
- 338 - 346 (9page)
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초록· 키워드
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Two molecular epidemiologic methods, IS6110 restriction fragment length polymorphism (IS6110-RFLP) and 24-locus mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR), are used worldwide in studies of Mycobacterium tuberculosis (MTB). Conversely, because of its poor resolution, pulsed-field gel electrophoresis (PFGE) is not widely used for MTB. In this study, we improved the 24-locus MIRU-VNTR and PFGE protocols and compared the effectiveness of these approaches for the molecular typing of MTB using 75 clinical isolates obtained from a cohort investigation of high-risk populations infected with MTB. The 24-locus MIRU-VNTR method demonstrated superior discriminatory ability, followed by PFGE and IS6110-RFLP. Next, we analyzed six isolates with clear epidemiologic connections; that is, isolates from patients who attended the same school. IS6110-RFLP and PFGE identified these samples as the same type. By contrast, according to MIRU-VNTR, two isolates differed from four other isolates at one locus each; one isolate was identified as Mtub29 and the other as QUB-26. In summary, the 24-locus MIRU-VNTR assay was the most useful molecular typing method among the three methods investigated due to its discriminatory power, short time required, and availability as an epidemiologic investigation tool. PFGE was the second-best method. Compared with the other loci assessed in the 24-locus MIRU-VNTR assay, the Mtub29 and QUB-26 loci appeared to exhibit greater variability during transmission.
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참고문헌 (21)
참고문헌 신청
Khosravi AD / 2014 / Application of pulsed field gel electrophoresis for study of genetic diversity in Mycobacterium tuberculosis strains isolated from tuberculosis patients / Jundishapur J. Microbiol. 7:e9963
Sevilla I / 2007 / Pulsed-field gel electrophoresis profile homogeneity of Mycobacterium avium subsp. paratuberculosis isolates from cattle and heterogeneity of those from sheep and goats / BMC Microbiol. 7:18
Matsumoto CK / 2011 / Diversity of pulsed-field gel electrophoresis patterns of Mycobacterium abscessus type 2 clinical isolates / J. Clin. Microbiol. 49:62~68
Iakhiaeva E / 2013 / Mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) genotyping of Mycobacterium intracellulare for strain comparison with establishment of a PCR-based database / J. Clin. Microbiol. 51:409~416
Jeon SM / 2014 / Analysis of species and intra-species associations between the Mycobacterium abscessus complex strains using pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) / J. Microbiol. Methods 104:19~25
Sevilla I / 2007 / Pulsed-field gel electrophoresis profile homogeneity of Mycobacterium avium subsp. paratuberculosis isolates from cattle and heterogeneity of those from sheep and goats / BMC Microbiol. 7:18
Matsumoto CK / 2011 / Diversity of pulsed-field gel electrophoresis patterns of Mycobacterium abscessus type 2 clinical isolates / J. Clin. Microbiol. 49:62~68
Iakhiaeva E / 2013 / Mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) genotyping of Mycobacterium intracellulare for strain comparison with establishment of a PCR-based database / J. Clin. Microbiol. 51:409~416
Jeon SM / 2014 / Analysis of species and intra-species associations between the Mycobacterium abscessus complex strains using pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) / J. Microbiol. Methods 104:19~25
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