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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제9호
- 발행연도
- 2010.1
- 수록면
- 1,314 - 1,321 (8page)
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초록· 키워드
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Sulfur metabolism in S. cerevisiae is well established, but the mechanisms underlying the formation of sulfide remain obscure. Here, we investigated by real-time RTPCR the dependence of expression levels of MET3, MET5/ECM17, MET10, MET16, and MET17 along with SSU1 on nitrogen availability in two wine yeast strains that produce divergent sulfide profiles. MET3 was the most highly expressed of the genes studied in strain PYCC4072, and SSU1 in strain UCD522. The strains behaved differently according to the sampling times, with UCD522 and PYCC4072 showing the highest expression levels at 120 h and 72 h, respectively. In the presence of 267 mg assimilable N/l, the genes were more highly expressed in strain UCD522 than in PYCC4072. MET5/ECM17 and MET17 were only weakly expressed in both strains under any condition tested. MET10 and SSU1 in both strains,but MET16 only in PYCC4072, were consistently upregulated when sulfide production was inhibited. This study illustrates that strain genotype could be important in determining enzyme activities and therefore the rate of sulfide liberation. This linkage, for some yeast strains, of sulfide production to expression levels of genes associated with sulfate assimilation and sulfur amino acid biosynthesis could be relevant for defining new strategies for the genetic improvement of wine yeasts.
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참고문헌 (23)
참고문헌 신청
/ 1990 / Office International de la Vigne et du Vin Recueil des methodes internationales d’analyse des vins et des mouts / OIV
Acree, T. E / 1971 / Determination of hydrogen sulfide in fermentation broths containing SO2 / Appl. Microbiol 22 : 110 ~ 112
Aranda, A / 2006 / Sulfur and adenine metabolisms are linked, and both modulate sulfite resistance in wine yeast / J. Agric. Food Chem 54 : 5839 ~ 5846
Bohlscheid, J. C / 2007 / The influence of nitrogen and biotin interaction on the performance of Saccharomyces in alcoholic fermentation / J. Appl. Microbiol 102 : 390 ~ 400
Carrasco, P / 2003 / Arginase activity is a useful marker for nitrogen limitation during alcoholic fermentation / Syst. Appl. Microbiol 26 : 471 ~ 479
Acree, T. E / 1971 / Determination of hydrogen sulfide in fermentation broths containing SO2 / Appl. Microbiol 22 : 110 ~ 112
Aranda, A / 2006 / Sulfur and adenine metabolisms are linked, and both modulate sulfite resistance in wine yeast / J. Agric. Food Chem 54 : 5839 ~ 5846
Bohlscheid, J. C / 2007 / The influence of nitrogen and biotin interaction on the performance of Saccharomyces in alcoholic fermentation / J. Appl. Microbiol 102 : 390 ~ 400
Carrasco, P / 2003 / Arginase activity is a useful marker for nitrogen limitation during alcoholic fermentation / Syst. Appl. Microbiol 26 : 471 ~ 479
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