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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제1호
- 발행연도
- 2008.1
- 수록면
- 153 - 159 (7page)
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초록· 키워드
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The limitations in current technology for generating
transgenic animals, such as the time and the expense,
hampered its extensive use in recombinant protein
production for therapeutic purpose. In this report, we
present a simple and less expensive alternative by directly
infusing a recombinant adenovirus vector carrying human
lactoferrin cDNA into rabbit mammary glands. The milk
serum was collected from the infected mammary gland
48 h post-infection and subjected to a 10% SDS-PAGE
and Western blotting. An 80-kDa protein was visualized
after viral vector infection. With this method, we obtained
a high level of expressed human lactoferrin of up to
2.3 mg/ml in the milk. Taken together, the method is
useful for the transient high-level expression recombinant
proteins, and the approach established here is probably one
of the most economical and efficient ways for large-scale
production of recombinant proteins of biopharmaceutical
interest.
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참고문헌 (23)
참고문헌 신청
Archer, J. S. / 1994 / Human growth hormone (hGH) secretion in milk of goats after direct transfer of the hGH gene into the mammary gland by using replication-defective retro
Arnold, R. R. / 1977 / A bactericidal effect for human lactoferrin / Science 197 : 263 ~ 265
BAE, GUN WON / 2006 / High Productivity of t-PA in CHO Cells Using Hypoxia Response Element / Journal of Microbiology and Biotechnology 16 (5) : 695 ~ 703
Bellamy, W. / 1992 / Antibacterial spectrum of lactoferricin B, a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin. J. Appl / Bacteriol 73
Crouch, S. P. / 1992 / Regulation of cytokine release from mononuclear cells by the iron-binding protein lactoferrin / Blood 80 : 235 ~ 240
Arnold, R. R. / 1977 / A bactericidal effect for human lactoferrin / Science 197 : 263 ~ 265
BAE, GUN WON / 2006 / High Productivity of t-PA in CHO Cells Using Hypoxia Response Element / Journal of Microbiology and Biotechnology 16 (5) : 695 ~ 703
Bellamy, W. / 1992 / Antibacterial spectrum of lactoferricin B, a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin. J. Appl / Bacteriol 73
Crouch, S. P. / 1992 / Regulation of cytokine release from mononuclear cells by the iron-binding protein lactoferrin / Blood 80 : 235 ~ 240
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