지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The purpose of this study was to express bovine lactoferrin N-lobe in Chlorella vulgaris, a green microalga, using the pCAMBIA1304 vector. Chlorella-codon-optimized bovine lactoferrin N-lobe (Lfb-N gene) was cloned in the expression vector pCAMBIA1304, creating the plasmid pCAMLfb-N. pCAMLfb-N was then introduced into C. vulgaris by electro-transformation. Transformants were separated from BG-11 plates containing 20 ${\mu}g\;mL^{-1}$ hygromycin. Polymerase chain reaction was used to screen transformants harboring Lfb-N gene. Finally, total soluble protein was extracted from the transformants, and the expression of Lfb-N protein was detected using western blotting. Using this method, we successfully expressed bovine lactoferrin in C. vulgaris. Therefore, our results suggested that recombinant lactoferrin N-lobe, which has many uses in the biomedical and pharmaceutical industries, can be produced economically.
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참고문헌 (55)
참고문헌 신청
Aisen, P. / 1980 / Iron transport and storage proteins / Annu. Rev. Biochem 49:357~393
Akhond, M. A. Y. / 2009 / Biotech crops: technologies, achievements and prospects / Euphytica 166:47~59
Azencott, H. R. / 2007 / Influence of the cell wall on intracellular delivery to algal cells by electroporation and sonication / Ultrasound Med. Biol 33:1805~1817
Babinger, P. / 2001 / A link between DNA methylation and epigenetic silencing in transgenic Volvox carteri / Nucleic Acids Res 29:1261~1271
Boynton, J. E. / 1993 / Chloroplast transformation in Chlamydomonas / Methods Enzymol 217:510~536
Akhond, M. A. Y. / 2009 / Biotech crops: technologies, achievements and prospects / Euphytica 166:47~59
Azencott, H. R. / 2007 / Influence of the cell wall on intracellular delivery to algal cells by electroporation and sonication / Ultrasound Med. Biol 33:1805~1817
Babinger, P. / 2001 / A link between DNA methylation and epigenetic silencing in transgenic Volvox carteri / Nucleic Acids Res 29:1261~1271
Boynton, J. E. / 1993 / Chloroplast transformation in Chlamydomonas / Methods Enzymol 217:510~536
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