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lactic acid bacteria (LAB) that produce bile salt hydrolase(BSH), and to evaluate its effects on serum cholesterol level.One-hundred-twenty bacterial colonies were initially isolatedfrom human feces, and five strains were selected after screeningbased on their resistance to acids, tolerance against bile salts,and inhibitory activity on Escherichia coli. The Lactobacillusplantarum strain with the highest level of BSH activity wasidentified using 16S rRNA sequences, and was named L.plantarum CK 102. L. plantarum108 cfu/ml survived in pH 2 bufer for 6 h and exhibitedexcellent tolerance for bile salt. Coculturing the strain with E.coli in MRS broth resulted in strong inhibition against growthof E. coli at 18 h. Furthermore, the potential effect of CK 102on serum cholesterol level was evaluated in rats. Thirty-tworats [Sprague-Dawley (SD) male, 129±1 g, 5 weeks old] weredivided into four groups of eight each. For six weeks, Group 1was fed a normal diet (negative control); Group 2 was fed aa cholesterol-enriched diet plus L. plantarum CK 102 at 1.0×107 cfu/ml; and Group 4 was fed a cholesterol-enriched dietplus L. plantarum CK 102 at 5.0×107cfu/ml. Blood samples werecollected, serum lipids were analyzed, and weights of the organswere measured. Total blood cholesterol level, triglyceride, LDL-cholesterol, and free-cholesterol values were lower in rats that werefed L. plantarum CK 102 than in those not fed L. plantarumCK 102. This cholesterol lowering effect implies that L. plantarumCK 102 could be utilized as an additive for health-asistancefoods. In conclusion, these results suggest that the L. plantarumCK 102 isolated could be used comercialy as a probiotic.

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